基质金属蛋白酶和组织金属蛋白酶抑制剂在肾细胞癌组织中的表达  

Expression of Tissue levels of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases in Renal cell Carcinoma

作　　者：杨亮[1] 乔振奎[2] 邴建勇[1] 奚晓龙[1] 朴庆华[1] 

机构地区：[1]大庆油田总医院泌尿外科,黑龙江大庆163500 [2]哈尔滨医科大学附属第四医院泌尿外科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2014年第13期2446-2449,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12511228)

摘　　要：目的:基质金属蛋白酶及组织金属蛋白酶抑制剂在肾细胞癌转移中占有重要的作用,研究肾细胞癌组织中MMP-2、MMP-9、TIMP-1和TIMP-2的表达情况,为肾癌转移的治疗提供理论依据。方法:选取36例肾细胞癌肾组织标本,从相同的肾细胞癌组织及癌旁肾组织获得对照样本,均进行根治性肾切除手术切除。肿瘤分期按TNM分期标准。为了统计评估,肿瘤1期和2期为低级,3期以上为高级。RT-PCR检测肿瘤和正常组织中的MMP-2、MMP-9、TIMP-1和TIMP-2的表达。结果:不同样本MMPs和TIMPs表达水平各不相同。肾细胞癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2在肾细胞癌中的表达明显高于正常肾组织(P<0.05)。在肾细胞癌的肿瘤分期方面,MMP-2与MMP-9和肿瘤的分期显著相关,随着肿瘤分期的增加,MMP-2与MMP-9的表达明显升高(P<0.05),而TIMP-1与TIMP-2与肿瘤的分期无关。结论:肾细胞癌组织中TIMP-2、MMP-2,MMP-9,TIMP-1的mRNA表达显著高于正常肾组织,抑制MMPS的表达将成为治疗肾细胞癌转移的新的方向。Objective： Matrix metalloproteinases and tissue inhibitors of metalloproteinases play an important role in renal cell carcinoma. To examine the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in renal tissue samples of renal cell cancer, and To provide a theoretical basis for the treatment of renal cell carcinoma metastasis Methods： 36 cases of renal cell carcinoma specimens and normal samples were fully obtained from the same renal cell carcinoma tissue and tumor adjacent renal tissues, those were performed radical resection operation resection of kidney. Tumor staging according to TNM staging. For statistical evaluation, tumor 1 and 2 stage were determined lower grade, tumor 3 and senior stage were determined higher grade. Semi quantitative reverse transcription polymerase chain reaction（RT-PCR） to detect the expression of tumor and normal tissues of MMP-2, MMP-9, TIMP-1 and TIMP-2. Results： MMPs and TIMPs expression levels in different samples was different. Mean MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than those in the normal renal tissue（P〈0.05）. In renal cell cancer staging, MMP-2 and MMP-9 and tumor stage was significantly correlated with the increase of staging of tumor, MMP-2 and MMP-9 mRNA expression increased significantly with the TNM stage of the tumor（P〈0.05）. Conclusion： Mean MMP-2, MMP-9, TIMP-1 and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than in the normal renal tissue, the inhibition of MMPS expression will become the treatment of renal cell carcinoma metastatic to the new direction.

关 键 词：基质金属蛋白酶 组织金属蛋白酶抑制剂 肾细胞癌 实时定量逆转录聚合酶反应 

分 类 号：R737.11[医药卫生—肿瘤]