磷对地下水反硝化系统中细菌菌群结构的影响  

Effect of phosphorous on bacteria community structure of denitrification-based groundwater remediation systems

作　　者：张建美[1] 郝春博[2] 冯传平[2] 李思远[2] 张丽娜[2] 郝会玲[2] 朱江 

机构地区：[1]油气资源与勘探技术教育部重点实验室长江大学地球环境与水资源学院,湖北武汉430100 [2]中国地质大学(北京)水资源与环境学院,北京100083 [3]秦皇岛市引青管理局,河北秦皇岛066001

出　　处：《地学前缘》2014年第4期191-198,共8页Earth Science Frontiers

基　　金：国家“十一五”重点项目(2006BAJ08B04);国家自然科学基金项目(41371464);湿地生态与农业利用教育部工程研究中心开放基金项目

摘　　要：采用16SrDNA克隆文库法研究了两根磷矿石含量不同的反硝化柱中细菌菌群结构的异同,每根反硝化柱中的样品随机测定了108个克隆子序列。研究结果表明,尽管两反硝化柱内的细菌均具有高度多样性,但是磷矿石的投加与否可明显引起菌群结构的变化。无磷矿石添加的反硝化柱内细菌分属14个类群,而有磷矿石添加的反硝化柱内细菌分属10个类群。两反硝化柱内的优势菌群均为β-proteobacteria和Bacte-roidetes,其相对含量存在着一定的差别,添加磷矿石的反硝化柱样品中β-proteobacteria和Bacteroidetes在文库中所占的比例分别为50.93%、15.74%,而无磷矿石添加的反硝化柱样品中β-proteobacteria和Bacteroidetes在文库中所占的比例分别为49.07%、12.04%。此外,β-proteobacteria类细菌内Rhodocyclales类菌属占据主导地位,包括Thauera sp.、Azospira restricta、Denitratisoma等具有反硝化功能的菌属。而且,Rhodocyclales类细菌在PC柱样品文库中所占的比例比其在ZC柱样品文库中的比例高9.2%。因此,磷矿石作为反硝化柱的固相磷源,可以影响细菌菌群结构,增加优势菌群的数量,最终提高硝酸盐的去除效率。Using 16SrDNA clone library methods,the composition and structure of bacterial community in two denitrification-based columns with different quantity of phosphate rock were investigated.108clones were randomly selected and sequenced from both 16SrDNA libraries,individually.The results indicated that,although the composition of bacterial community in each column showed high diversity,the community structure was obviously different in each column under the different addition of phosphate rock.The bacterial communities in column without phosphate rock could be divided into 14groups of structure,while 10groups of bacterial communities were present in the column containing phosphate rock.The dominant bacterial communities wereβ-proteobacteria and Bacteroidetes in each column,and their contents showed some differences;the portions ofβ-proteobacteria and Bacteroidetes in library from column containing phosphate rock were 50.93% and 15.74%,respectively,and in the library of column without phosphate rock,the portions ofβ-proteobacteria and Bacteroidetes were 49.07%and 12.04%,respectively.Furthermore,Rhodocyclales were predominant in theβ-proteobacteria,including Thauera sp.,Azospira restricta,Denitratisoma,etc;which were capable of denitrifying.Moreover,the proportion of Rhodocyclales in the clone library from PC column was 9.2percent higher than that in the clone library from ZC.Consequently,the phosphate rock,as the excellent solid phosphorus source of denitrification-based groundwater remediation systems,positively affected the structure of bacterial communities,increased the number of predominant bacterium and eventually improved the nitrate removal efficiency.

关 键 词：反硝化细菌 磷矿石 16SrDNA克隆文库 细菌群落 

分 类 号：X172[环境科学与工程—环境科学]