帕瑞昔布对非小细胞肺癌细胞株A549增殖和迁移的影响  

Effect of parecoxib on proliferation and movement in non-small cell lung cancer cell line A549

作　　者：刘晨辉[1] 鲍红光[1] 葛亚力[1] 王书奎[1] 王晓亮[1] 沈妍[1] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)麻醉科,210006

出　　处：《中华临床医师杂志（电子版）》2013年第4期139-141,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：南京市医学科技发展重点项目(201108028)

摘　　要：目的研究帕瑞昔布对非小细胞肺癌A549细胞增殖和迁移的影响,并探讨其可能机制。方法采用随机数字表法将A549细胞随机分为四组:对照组(C组),10μmol/L帕瑞昔布(P1组)、40μmol/L帕瑞昔布组(P2组)和160μmol/L帕瑞昔布组(P3组)。C组细胞常规培养,P1、P2和P3组细胞分别用终浓度为10μmol/L、40μmol/L和160μmol/L的帕瑞昔布处理A549细胞24h。MTT法检测各组细胞的增殖情况,划痕试验检测各组细胞的迁移能力,Western blot法检测各组细胞p-AKT、survivin的表达情况。结果与C组比较,P1、P2和P3组细胞的增殖抑制率依次增高(P<0.05),P1、P2和P3组细胞的迁移距离依次降低(P<0.05),P1、P2和P3组细胞p-AKT和survivin的表达水平依次降低(P<0.05),且呈剂量依赖性。结论帕瑞昔布可以抑制A549细胞的增殖和迁移,其机制可能是抑制p-AKT和survivin的表达。Objective To study the effect of parecoxib on proliferation and movement in non-small cell lung cancer cell line A549. Methods The A549 ceils were randomly divided into 4 groups ： control group and different concentrations of parecoxib groups （P1-3 groups）. The cells in parecoxib groups were exposed to 10,40 and 160 μmol/L parecoxib for 24 h respectively. The rates of proliferation inhibition were analyzed via a MTI＂ assay, the distances of movement were detected by wound-healing experiment, and the expression of p-AKT and survivin protein were detected via Western blot analysis. Results Compared with C group, the rates of proliferation inhibition were significantly increased（ P 〈 0. 05 ）, the distances of movement were significantly decreased, and the expression of p- AKT and survivin proteins were significantly down-regulated（ P 〈 0. 05 ）in P1, P2 and P3 groups. All results showed a dose-dependent manner. Conclusions The parecoxib inhibits the proliferation and movement of A549 cells. This function of parecoxib may be related to the inhibition of AKT phosphorylation and down-regulation of survivin protein.

关 键 词：癌 非小细胞肺 细胞增殖 细胞运动 帕瑞昔布 

分 类 号：R734.2[医药卫生—肿瘤]