新型靶向光敏剂光动力诱导HeLa细胞凋亡的实验研究  

An Experimental Study on Apoptosis of HeLa Cells Induced by A New Targeting Photosensitizer-based Photodynamic Therapy(PDT)

作　　者：李鹏熙[1] 李毅[2] 李东红[1] 

机构地区：[1]创伤烧伤复合伤国家重点实验室,第三军医大学大坪医院野战外科研究所二室,重庆400042 [2]第三军医大学大坪医院肿瘤中心,重庆400042

出　　处：《肿瘤学杂志》2014年第5期383-387,共5页Journal of Chinese Oncology

基　　金：国家自然科学基金资助项目(21072227)

摘　　要：[目的]研究新型靶向光敏剂Ⅰ光动力诱导HeLa细胞死亡的方式。[方法]用流式细胞仪分析照射剂量和光敏剂浓度对HeLa细胞凋亡的影响,细胞凋亡和坏死的比例;并采用激光共聚焦观察细胞F-actin和核的变化;透射电镜观察细胞超微结构的改变。[结果]光敏剂Ⅰ介导的光动力治疗可诱导HeLa细胞的凋亡和坏死,但凋亡的比例明显大于坏死(P=0.0053),且细胞凋亡的量分别与光照剂量(r=0.987,P<0.001)和光敏剂浓度(r=0.995,P<0.001)呈正相关;光动力诱导后,细胞的F-actin结构破坏,细胞皱缩,透射电镜下可见凋亡小体的形成。[结论]光敏剂Ⅰ介导的光动力治疗主要通过凋亡途径导致HeLa细胞的死亡。[Purposel To study the death mode of HeLa cells induced by a new targeting photo- sensitizer I -based photodynamic therapy （PDI）. [Methods] The influence of irradiation dose and the concentration of photosensitizer I on the apoptosis of HeLa cells,and the percentage of apoptosis and necrosis of HeLa cells were analyzed by flow cytometry. The changes of F- actin and nuclear of HeLa cells were observed through confoca]. The changes of ultrastructure were observed under transmission electron microscope. [Results ] Compared with control cells, photosensitizer I -based PDT could induce apoptosis and necrosis in HeLa cells,but the percentage of apoptotic cells was obviously more than that of necrosis, and the number of apoptotic cells displayed positive correlation with the irradiation dose and the concentration of photosensitizer I ,the correlation coefficients were 0.987 （P〈0.O01） and 0.995 （P〈0.001） respectively. After PDT,the F-actin of HeLa cells was damaged, the nucleus shrank, and the apoptotic bodies could be observed under transmission electron microscope. [Conclusion] Apoptosis was the main death mode of HeLa cells induced by photosensitizer I -based PDT.

关 键 词：靶向性光敏剂I HELA细胞 光动力治疗 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]