CpG ODN对哮喘小鼠肺组织FcεRⅠ、FcγRⅡb表达的影响  

Effects of CpG ODN on FcεRⅠ and FcγRⅡb Expression in Asthma Model of Mice

作　　者：朱宝俊[1,2] 罗芳[3] 李孟荣[4] 

机构地区：[1]温州医科大学附属第二医院,325027 [2]浙江省舟山市普陀中医院,316100 [3]宁波市妇女儿童医院,315000 [4]温州医科大学附属第二医院育英儿童医院呼吸科哮喘及变态反应免疫治疗中心

出　　处：《医学研究杂志》2014年第5期89-92,共4页Journal of Medical Research

基　　金：温州市科技局对外科技合作交流项目(H20090021)

摘　　要：目的观察CpG ODN对哮喘小鼠肺组织FcεRⅠ、FcγRⅡb表达的影响,探讨其诱导免疫耐受的相关机制。方法将48只清洁级雄性Balb/c小鼠随机分为正常对照组(A组)、哮喘组(B组)、地塞米松(DXM)治疗组(C组)、CpG ODN治疗组(D组),每组12只。以卵白蛋白(OVA)作用建立小鼠哮喘模型。收集支气管肺泡灌洗液(bronchial alveolar lavage fluid,BALF)计数细胞总数并分类,取肺组织作病理,反转录-聚合酶链反应(RT-PCR)法测定肺组织中FcεRⅠ、FcγRⅡb mRNA的表达。结果 (1)电镜观察肺组织超微结构:B组显示支气管及血管周围有较多炎性细胞浸润,肺泡隔内嗜酸粒细胞浸润,C组和D组与B组相比明显减轻。(2)BALF中炎症细胞表达变化:与B组相比,C组、D组BALF中细胞总数、嗜酸性粒细胞(eosinophil,EOS)绝对值以及EOS占细胞总数的百分比(EOS%)均显著减少(P<0.01)。(3)RT-PCR结果:C组、D组FcεRⅠmRNA表达均显著低于B组(P<0.01),C组、D组FcγRⅡb mRNA表达均显著高于B组(P<0.01)。(4)相关分析:小鼠肺组织FcεRⅠ和FcγRⅡb表达呈显著负相关(P<0.01,n=40)。结论 CpG ODN能降低哮喘小鼠肺组织FcεRⅠ的表达,增加FcγRⅡb的表达,具有改善气道炎症和诱导免疫耐受的作用。Objective To investigate the possible mechanisms induced by CpG ODN on the expression of FcεRⅠ and FcγRⅡ b of lung tissue in acute asthma model of mice. Methods Totally 48 male Balb/c mice were randomly divided into the control group （group A） , asthma model group （group B） , dexamethasone control group （group C） , CpG ODN treatment group （group D） with 12 in each. Group B were sensitized by OVA to establish the acute mice asthma model. BALF were collected and the total cell numbers were counted. Lung were fixed and sections were used for Pulmonary uhrastructure. FcεRⅠ and FcγRⅡb mRNA in the lung were measured by RT - PCR. Results Pulmonary uhrastructure： Group B showed inflammation cell infiltration, pulmonary alveolus Ⅱ type epithelial cell swell- ing entry into the alveolar lumen. Group C and group D were much lighter than those of group B. In the BALF, compared with group B, the total cell numbers, the absolute numbers of EOS and EOS% were markedly decreased than group C and D （ P 〈 O. 01 ）. RT - PCR results ： The expression of FcεRⅠ in group D and group C were significantly lower than group B （ P 〈 0.01 ） , and the expression of FcγRⅡ b in group D and group C was significantly higher than group B （P 〈 0.01 ）. Correlative analysis： The expression of FcεRⅠ was negatively correlated with FcγRⅡ b （P 〈 0.01, n = 40）. Conclusion CpG ODN lower FcεRⅠ and increase FcγRⅡ b expression in the lung tissue of asthma mouse and improve airway inflammation, induce immune tolerance.

关 键 词：哮喘 小鼠 CPG ODN FcεRⅠ FcγRⅡb 免疫耐受 

分 类 号：R393[医药卫生—基础医学]