PTTG1影响胶质瘤细胞侵袭力机制的研究  

Effect of PTTG1 in the invasion of glioma cells

作　　者：陈为一[1] 李小龙[1] 齐岳亮 李洪利[2] 尹崇高[3] 刘晓丽[3] 张宝刚[1] 郭文君[1] 

机构地区：[1]潍坊医学院病理学教研室,山东潍坊261053 [2]潍坊医学院医学研究实验中心,山东潍坊261053 [3]潍坊医学院护理学院,山东潍坊261053

出　　处：《中国癌症杂志》2014年第5期329-332,共4页China Oncology

基　　金：国家自然科学基金(No:81072068);山东省中青年科学家科研奖励基金(No:2010BSB14050;BS2011YY060);山东省高等学校科技计划(No:J12LK03;J13LK03);潍坊医学院青年科技创新基金(No:K11QC1002)

摘　　要：背景与目的:研究证实垂体肿瘤转化基因1(pituitary tumor transforming gene 1,PTTG1)的表达情况与各类肿瘤细胞的恶性程度有关,但是其在胶质瘤中的作用尚不清楚。本研究旨在探讨PTTG1在恶性胶质瘤侵袭中的作用及其临床意义。方法:应用蛋白质印迹法(Western blot)检测PTTG1蛋白在不同胶质瘤细胞系中的表达;采用小RNA质粒干扰技术抑制U87细胞中PTTG1蛋白的表达,应用Western blot技术检测转染的效率;通过体外侵袭实验检测转染后细胞侵袭力的变化;采用Western blot技术检测经过表皮细胞生长因子(epithelial growth factor,EGF)刺激后敲除PTTG1的细胞组(siPTTG1/U87)与转染空载的细胞组(Scr/U87)中Akt、ARK5磷酸化的情况。结果:PTTG1蛋白在各恶性胶质瘤细胞系中均高表达;敲除PTTG1后U87细胞的侵袭力明显下降;对Scr/U87细胞进行EGF刺激5 min后,Akt、ARK5的磷酸化情况显著增强,而无论有无EGF的刺激,siPTTG1/U87中Akt、ARK5的磷酸化情况都没有明显改变。结论:在恶性胶质瘤细胞中,PTTG1蛋白呈高表达状态并且与侵袭性显著相关,其对侵袭性的调控可能是通过Akt-ARK5通路实现的。Background and purpose：Numerous researches indicated that the expression of pituitary tumor transforming gene1 （PTTG1） was correlated with the severity of glioma tumors. However the specific mechanism of PTTG1 is not clear in glioma. In this study, we explored the role and significance of PTTG1 in the invasion of glioma cells. Methods：Western blot was used to detect the expression of PTTG1 protein in various glioma cell lines. siRNA plasmid was used to transfect U87 cells. Western blot was used to analyze the expression of PTTG1 protein in transfected U87 cells. Matrigel invasion assay was used to detect the invasive ability in the cells being transfected in vitro. Western blot was used to analyze epithelial growth factor （EGF） induced protein phosphorylation of ARK5 and Akt in the cells being transfected PTTG1 plasmid （siPTTG1/U87） and scrambled siRNA （Scr/U87）. Results：The expression of PTTG1 protein was higher in all glioma cell lines. After transfection, the invasion of siPTTG1/U87 was obviously decreased after 5 min with EGF stimulation than the Scr/U87, the phosphorylation of ARK5 and Akt was significantly enhanced. However, whether or not the existence of EGF, the phosphorylation of ARK5 and Akt had no differences in siPTTG1/U87. Conclusion：In glioma cells, PTTG1 protein is high expressed and maybe have an important function in glioma cells invasion through Akt-ARK5 signaling pathway.

关 键 词：胶质瘤 侵袭 垂体肿瘤转化基因1 ARK5 SIRNA 

分 类 号：R739.41[医药卫生—肿瘤]