不同方法接种构建的SCID小鼠HL-60白血病模型生物学特性分析  

Biological features of the mouse HL-60 leukemia models constructed by different inoculation methods

作　　者：单武林 章成芳 马筱玲[1] 常文娇[1] 戴媛媛[1] 

机构地区：[1]安徽医科大学附属省立医院检验科,合肥230001

出　　处：《安徽医科大学学报》2014年第6期837-841,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:11040606M205)

摘　　要：采用皮下(A组)及腹腔(B组)2种途径将HL-60细胞接种于经环磷酰胺预处理的SCID小鼠。C组小鼠不做处理作为正常对照。比较不同接种方法构建的小鼠HL-60白血病模型的生物学特性。结果表明皮下、腹腔注射培养的HL-60细胞于SCID小鼠体内均可成瘤。皮下注射成瘤时间短,存活时间长;腹腔注射成瘤时间长,存活时间短。腹腔注射脏器浸润较皮下成瘤严重。接种细胞4周后,B组小鼠外周血白细胞数较A和C组明显升高,差异有统计学意义(P<0.05)。A和B两组小鼠外周血都有白血病细胞浸润,但是比例较低,不超过3%。HL-60 cells were conventionally inoculated into each cyclophosphamide pretreated SCID mouse via sub-cutaneous injections （ A group） , intraperitoneal injections （ B group） . Mice in C group were untreated and used as the control group. Then the biological characteristics of mouse HL-60 leukemia models inoculated differently were investigated. The results showed that both two inoculation methods were feasible for developing mouse leukemia models. Compared to A group, the duration of tumor formation was longer and the survival time of mice was shorter in B group. However, organs infiltration was more obvious in B group. There was statistically significant difference in the number of leukocytes among the peripheral blood between A and B groups from the forth week. Leukemic cells could also be found in peripheral blood in A and B groups, but the percent was low, not more than 3%.

关 键 词：急性白血病 SCID 模型 皮下 腹腔 

分 类 号：R733.71[医药卫生—肿瘤]