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作 者:申阳[1] 邵江华[2] 彭小刚[3] 曹青[3] 刘秀霞[3] 贺文凤[3] 刘欣[1] 洪葵[1]
机构地区:[1]南昌大学第二附属医院心内科,南昌330006 [2]南昌大学第二附属医院普外科 [3]江西省分子医学重点实验室
出 处:《临床心血管病杂志》2014年第5期447-450,共4页Journal of Clinical Cardiology
基 金:教育部创新团队基金(No:IRT1141);国家自然科学基金(No:81070148;81060196);国家重大基础研究973计划(No:2007CB512002;2008CB517305)
摘 要:目的:确定FAT10在心肌细胞的表达及其在细胞凋亡中发挥的作用。方法:①提取大鼠、小鼠和人心脏组织总RNA与总蛋白,分别用RT-PCR与Western Blot检测FAT10的表达。②构建大鼠心肌梗死(心梗)模型与大鼠乳鼠原代心肌细胞缺氧/复氧(H/R)模型,分别用实时荧光定量PCR与Western Blot检测FAT10的表达变化。③体外构建FAT10过表达慢病毒,转染原代心肌细胞后暴露于H/R的环境中,通过流式细胞术检测心肌细胞凋亡率,用Western Blot检测凋亡相关蛋白Bcl-2与BAX的表达量。结果:FAT10的mRNA与蛋白在大鼠、小鼠和人的正常心脏组织中均有表达。在大鼠心梗模型的梗死心肌边缘区与H/R处理的大鼠原代心肌细胞中,FAT10表达量明显升高。在原代心肌细胞中过表达FAT10可以降低H/R引起的细胞凋亡,并且升高抗凋亡蛋白Bcl-2的表达,下调促凋亡蛋白BAX的表达。结论:FAT10是心肌组织表达的新蛋白,具有心脏保护作用,FAT10的表达上调能对抗心肌细胞凋亡。Objective:To determine the expression of FAT10in cardiac myocyte and the role of FAT10in apoptosis.Method:①Expression of FAT10in normal human and murine hearts was detected by RT-PCR and Western Blot.②Using myocardial infarction model in rats and cultured neonatal rat cardiac myocytes(NRCM) subjected to hypoxia/reoxygenation(H/R)stress,the change of FAT10expression was assessed by qRT-PCR and Western Blot.③After lentiviral-mediated overexpression of FAT10in NRCM,the apoptosis was assayed by flow cytometry and BCL-2,BAX expression level was also detected.Result:FAT10 was expressed in normal human and murine hearts.Lentiviral-mediated overexpression of FAT10in NRCM was associated with reduced apoptosis in response to H/R injury.Expression of FAT10was associated with increased anti-apoptotic protein Bcl-2level and reduced pro-apoptotic protein BAX level.Conclusion:FAT10is expressed in cardiac myocytes. Upregulation of FAT10protects cardiac myocytes against apoptosis by shifting the Bcl-2/Bax against apoptosis. Thus,FAT10is a cardioprotective gene and its upregulation might afford beneficial effects in pathological conditions associated with cardiac myocyte apoptosis.
关 键 词:FAT10 类泛素蛋白 心肌细胞 缺氧 细胞凋亡
分 类 号:R541.4[医药卫生—心血管疾病]
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