去卵巢对大鼠牙槽骨骨质量及实验性牙周炎的影响  

Impact of ovariectomy on the mandibular alveolar bone mineral density and experimental periodontitis

作　　者：颜承伟 安晶涛[2] 李莹[3] 张斌[3] 金星爱[1] 刘英群[1] 

机构地区：[1]哈尔滨医科大学附属第一医院儿童口腔科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院口腔正畸科,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第二医院口腔科,黑龙江哈尔滨150086

出　　处：《牙体牙髓牙周病学杂志》2014年第5期282-285,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81170960)

摘　　要：目的:探讨切除卵巢后,大鼠牙槽骨骨质的变化情况及其对实验性牙周炎进展的影响。方法:取3月龄健康雌性Wistar大鼠20只,随机分为2组(n=10),分别进行卵巢切除(卵巢切除组,OVX)和卵巢周围脂肪组织切除(假手术组,SHAM);3个月后在所有大鼠下颌右侧第一磨牙(M1)牙颈部结扎丝线诱导牙周炎。于切除卵巢后第5个月处死各大鼠,显微CT测量骨形态学参数,并于三维重建模型上测量牙槽骨高度丧失程度。结果:术后5个月,OVX组大鼠牙槽骨的平均骨密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)均明显低于SHAM组(P<0.05);骨表面积和体积比(BS/BV)、骨小梁分离度(Tb.Sp)均明显高于SHAM组(P<0.05);OVX组大鼠的牙槽骨高度丧失程度与SHAM组相比无明显差异(P>0.05)。结论:大鼠去除卵巢后牙槽骨发生骨质疏松,但并不加重实验性牙周炎所致的牙槽骨高度丧失。AIM ： To investigate the impact of ovariectomy on mandibular alveolar bone mineral density and periodontal disease. METHODS：Twenty 12 -week -old female Wistar rats were randomly assigned into sham surgery （SHAM） group and ovariection（OVX） group （ n = 10）. 3 months after surgery, periodontal disease was induced by silk ligature around the neck of the mandibular right first molar for 2 months. Then the rats were sacrificed, Micro - CT scan was carried out to detect the bone mineral density （BMD）, trabecular thickness （Tb. Th） , bone volume fraction （BV/TV） , trabecular separation （Tb. Sp. ） and bone surface to bone volume （BS/BV） of mandibular alveolar bone. Alveolar bone height loss （ABHL） was measured on 3D models. RESULTS： 5 months after ovariectomy, the values BMD, Tb. Th and BV/TV in OVX rats were lower than those in SHAM rats （ P 〈 0. 05 ） , whereas the values of Tb. Sp and BS/BV of the OVX rats increased compared with SHAM rats （ P 〈 0.05 ）. However, no difference was found in ABHL between 2 groups. CONCLUSION： Ovariectomy induces osteoporosis in the mandibular alveolar bone of rats, but has no effect on the alveolar bone height loss of rats with experimental periodontitis.

关 键 词：卵巢切除术 牙槽骨丧失 实验性牙周炎 显微CT 

分 类 号：R781.4[医药卫生—口腔医学]