沉默CCL19对结直肠癌增殖、迁移、侵袭和促血管形成的影响  

Effects of silencing CCL19 on migration, invasion, and proangiopoiesis of colorectal cancer cells

作　　者：陆军[1,2] 赵敬坤[1,2] 陆爱国[1] 冯浩 王蒲雄志[1] 张卓[1] 宗雅萍[1] 马君俊[1] 刘炳亚[2] 郑民华[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院微创外科,上海200025 [2]上海交通大学医学院附属瑞金医院上海市消化外科研究所,上海200025 [3]慕尼黑大学医学院,德国慕尼黑80539

出　　处：《上海交通大学学报（医学版）》2014年第5期578-583,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81201625);上海市科委引导项目(124119a0900)~~

摘　　要：目的研究趋化因子CCL19在结直肠肿瘤中的作用。方法采用Real—TimePCR和Westernblotting技术筛选高表达CCL19的细胞株SW620细胞作为研究对象，并用siRNA沉默SW620中的CCL19基因。分别采用细胞增殖实验、Transwell迁移、侵袭实验和小管形成实验来检测SW620细胞的增殖、迁移、侵袭和促血管形成能力的变化。结果Westernblotting和Real—TimePCR证实SW620中CCL19相对其他细胞株高表达。经siRNA-CCL19干扰后，SW620细胞在48～120h内细胞增殖能力显著上升（P〈0．05），细胞迁移、侵袭能力亦明显上升（P〈0．05），促血管形成能力明显增强。结论SW620是相对高表达CCL19的细胞株，沉默CCL19基因后可以明显促进SW620细胞在体外的增殖、迁移、侵袭和促血管形成能力，因此认为CCL19在结直肠癌发展中起着抑制作用，并有应用于抗癌药物研究的前景。Objective To investigate the role of chemokine CCL19 in colorectal carcinoma tissues. Methods SW620 Cell line with highest expression level of CCL19 was screened for the study by the Real-Time quantitative PCR and Western blot. CCL19 of SW620 was silenced by siRNA. The changes of proliferation, migration and invasion, and proangiopoiesis abilities of SW620 were detected by the CCK-8, Transwell, and tube formation assay, respectively. Results The Western blotting and Real-Time PCR confirmed that the expression of CCL19 in SW620 was higher than that of other cell lines. The proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells within 48 -120 h were significantly improved after CCL19 being silenced. Conclusion The expression level of CCL19 in SW620 is relatively high. Silencing CCL19 can significantly enhance the proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells in vitro, so it is believed that the CCL19 can inhibit the development of colorectal cancer and is potential to be applied to the research of anticancer drugs.

关 键 词：趋化因子CCL19 细胞增殖 细胞迁移 细胞侵袭 血管形成 

分 类 号：R735.34[医药卫生—肿瘤]