三氟拉嗪对人子宫内膜癌Ishikawa细胞的抑制作用  

Inhibitory effect of trifluoperazine on Ishikawa cells of human endometrial carcinoma

作　　者：郑静[1] 孙笑[2] 王晶[2] 王丽华[2] 王玉东[2] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院麻醉科,上海200030 [2]上海交通大学医学院附属国际和平妇幼保健院妇科,上海200030

出　　处：《上海交通大学学报（医学版）》2014年第5期609-612,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81172477);上海市科委自然科学基金(11ZR1440800;13JC1401303);上海市新百人计划(XBR2013097)~~

摘　　要：目的观察盐酸三氟拉嗪(TFP)在体外对人子宫内膜癌Ishikawa细胞生长和增殖的影响,探讨可能的作用机制。方法采用MTT法检测不同浓度TFP体外干预对Ishikawa细胞生长的抑制作用,计算药物的半抑制浓度(IC50)并选择最佳作用时间。流式细胞仪分析TFP对Ishikawa细胞周期的影响;Real-Time PCR检测TFP对Ishikawa细胞内叉头框转录因子O亚族1(FOXO1)和抑癌基因Kruppel样因子6(KLF6)表达的调控作用。结果不同浓度TFP对Ishikawa细胞的生长均有抑制作用,呈剂量和时间依赖性,IC50为16.56μmol/L;20μmol/L作用24 h的抑制效果最佳。20μmol/L TFP干预Ishikawa细胞24 h,S期细胞比例明显降低(P<0.01),KLF6 mRNA表达显著上调(P<0.05)。结论三氟拉嗪体外干预可抑制子宫内膜癌Ishikawa细胞的生长和增殖,其作用机制可能与抑癌基因KLF6表达上调有关。Objective To explore the effects of trifluoperazine （TFP） on the growth and proliferation of Ishikawa cells of human endometrial carcinoma in vitro and their possible mechanisms. Methods The inhibitory effect of TFP of different concentrations on the growth of Ishikawa cells of human endometrial carcinoma was detected by the MTT assay. The half maximal inhibitory concentration （IC50） of the drug was calculated and the optimal time was selected. The effect of TFP on the cell cycle of Ishikawa cells was measured by the flow cytometer. The regulatory effect of TFP on the expressions of forkhead box transcription factor O1 （FOXO1） and Kruppel-like factor 6 （KLF6） in Ishikawa cells was determined by the Real-Time PCR. Results TFP of different concentrations would inhibit the growth of Ishikawa cells. The inhibitory effect depended on the dose and time. The IC50 value of TFP was 16.56μmol/L. The optimal time was 24 h for being treated by TFP of 20 μmol/L. After Ishikawa cells were treated by TFP of 20 μmol/L for 24 h, the proportion of S phase cells was significantly decreased （ P 〈 0.01 ） and the expression of KLF6 mRNA was siguificandy up-regulated （P〈0.05）. Conclusion The intervention of TFP in vitro can inhibit the growth and proliferation of Ishikawa cells of endometrial carcinoma. The mechanism may be relevant to up-regulate the expression of the tumor suppressor gene KLFt.

关 键 词：子宫内膜癌 三氟拉嗪 细胞生长 细胞增殖 Kruppel样因子6 

分 类 号：R737.33[医药卫生—肿瘤]