极低频电磁场对人胚胎眼巩膜成纤维细胞中ROCK1及COL1A1表达的调控作用  

Effect of extremely low frequency electromagnetic fields on expression of ROCK1 and COL1A1 in human fetal sclera fibroblasts

作　　者：牛宗镇[1] 朱煌[1] 蔡晓静[1] 王洁[1] 冯婕妤[1] 

机构地区：[1]上海交通大学医学院附属新华医院眼科,上海200092

出　　处：《上海交通大学学报（医学版）》2014年第5期640-644,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的观察极低频电磁场(ELF-EMFs)对人胚胎眼巩膜成纤维细胞(HFSFs)中Rho相关的卷曲蛋白激酶1(ROCK1)及Ⅰ型胶原蛋白α1链(COL1A1)表达的影响。方法 HFSFs体外培养和传代后以0.2 mT、50 Hz电磁场辐射24 h(暴露组),设立未行电磁场辐射的HFSFs作为对照组。Real-Time PCR检测ROCK1和COL1A1 mRNA的表达,Western blotting检测ROCK1和COL1A1蛋白的表达。结果 Real-Time PCR检测结果显示:与对照组相比,暴露组HFSFs中ROCK1 mRNA的表达显著上调(t=3.006,P=0.039 7),而COL1A1mRNA的表达显著下调(t=4.225,P=0.013 4)。Western blotting检测发现,暴露组HFSFs中ROCK1蛋白的表达升高,COL1A1蛋白的表达降低。结论 ELF-EMFs对HFSFs中ROCK1和COL1A1的表达具有调控作用,并因此使巩膜重塑,进而导致近视的发生和发展。Objective To investigate the effects of extremely low frequency electromagnetic fields （ELF-EMFs） on the expression of Rho-associated coiled-coil protein kinase 1 （ROCK1） and COL1A1 in human fetal sclera fibroblasts. Methods HFSFs were cultured and passaged in vitro and divided into the exposure group which was exposed to the electromagnetic field of 0.2 mT and 50 Hz for 24 h, and the control group which was not exposed to the electromagnetic field. The expressions of ROCK1 and COL1A1 mRNA were detected by the Real-Time quantitative polymerase chain reaction （ Real-Time PCR） and the expression levels of ROCK1 and COL1A1 proteins were detected by the Western blotting. Results The results of the Real-Time PGR showed that compared to the control group, the expression of ROCK1 mRNA in HFSFs of the exposure group was significantly up-regulated （ t =3. 006, P =0. 0397）, while the expression of COL1A1 mRNA was significantly down-regulated （t=4. 225, P =0. 0134）. The results of the Western blotting indicated that the expression of ROCK1 protein in HFSFs of the exposure group was increased and the expression of COL1A1 protein was decreased. Conclusion ELF-EMFs can regulate the expression of ROCK1 and COL1A1 in HFSFs and thus remodel the sclera and cause the occurrence and development of myopia.

关 键 词：极低频电磁场 人胚胎眼巩膜成纤维细胞 Rho相关的卷曲蛋白激酶1 Ⅰ型胶原蛋白 

分 类 号：R779.6[医药卫生—眼科]