FGF-23(R176Q)过表达对成年小鼠磨牙龋损的影响  

The effective of FGF-23( R176Q) overexpression on the caries development of adult mouse molar

作　　者：刘洪[1] 孙雯[2] 郑阳玉[2] 

机构地区：[1]盐城卫生职业技术学院,224005 [2]南京医科大学口腔医学研究所

出　　处：《实用口腔医学杂志》2014年第3期322-326,共5页Journal of Practical Stomatology

基　　金：盐城市医学科技发展计划项目(编号:YK2012029)

摘　　要：目的:研究FGF-23(R176Q)过表达对成年小鼠磨牙龋损的影响及机制。方法:使用CT扫描、血清学检查、HE染色、免疫组化染色和real time RT-PCR等方法检测分析FGF-23(R176Q)转基因小鼠(TG组,n=20)和野生型小鼠(WT组,n=20)血清钙磷浓度变化和下颌第一磨牙矿化的差异。结果:WT组小鼠血清钙、磷和1,25(OH)2D3浓度均明显高于TG组(P<0.05),PTH的浓度低于TG组(P<0.05);TG组下颌第一磨牙龋损率高于WT组(P<0.05),前期牙本质厚度高于WT组(P<0.05),而继发性牙本质面积低于WT组(P<0.05);TG组的Biglycan阳性百分比高于WT组(P<0.05),而DSP阳性百分比低于WT组(P<0.05);WT组的ALP和Ⅰ型胶原mRNA表达水平均明显高于TG组(P<0.05)。结论:FGF-23(R176Q)过表达能够抑制牙本质基质形成,减少前期牙本质形成和矿化,减少继发性牙本质形成,进而导致了龋损增加。Objective： To study the effects of FGF-23 （R176Q） overexpression on the caries development of adult mouse molar. Methods： CT scanning, serological examination, HE staining, immunohistochemical staining and real time RT-PCR analysis were used to compared the serum calcium, phosphorus concentrations and mandibular first molar mineralization of FGF-23 （R176Q） transgenic mice （TG group, n = 20 ） and wild type mice（ WT group, n = 20 ）. Results： Caries prevalence in TG group was higher than that in WT group （ P 〈 0.05 ）. Serum calcium, phosphorus and 1,25 （ OH ） 2 D3 concentrations in WT group were higher and PTH was lower than those in TG group（ P 〈 0.05 ） ; the predentin thickness of TG group was higher while the secondary dentin area was smaller than those of WT group （P 〈 0.05 ） ; the positive percentage of Biglycan of TG group was higher while the DSP positive percentage was lower than those of WT group（P 〈 0.05 ） ; the ALP and collagen I mRNA levels in the WT group were higher than those in TG group（P 〈 0.05）. Conclusion： FGF-23 （R176Q） overexpression can inhibit the formation of dentin matrix, reduce predentin mineralization, reduce the formation of secondary dentin and increase dental caries.

关 键 词：FGF-23 转基因 继发性牙本质 矿化 龋损 

分 类 号：R781.1[医药卫生—口腔医学]