腺苷脱氨酶影响了甲氨蝶呤对破骨细胞的抑制  

Adenosine deaminase affects methotrexate-induced suppression of osteoclastogenesis

作　　者：屈鹏飞[1] 陈彪[1] 杨威[1] 张凯[1] 崔颐[1] 张文娟[1] 范戌辉[1] 蒋崇槟[1] 张平[1] 

机构地区：[1]河北医科大学第二医院口腔颌面外科,石家庄050000

出　　处：《实用口腔医学杂志》2014年第3期375-378,共4页Journal of Practical Stomatology

基　　金：河北省科技厅课题(编号:132777137)

摘　　要：目的:研究腺苷脱氨酶(ADA)和甲氨蝶呤(MTX)对破骨细胞形成的作用。方法:用大鼠的全骨髓细胞培养体系(WBMCs)来评价破骨细胞形成。用半定量RT-PCR来评估MTX对ADA mRNA水平的影响。结果:全骨髓细胞培养系中,只有腺苷和脱氧腺苷消除了MTX对破骨细胞形成的抑制作用;MTX可抑制ADA mRNA的表达。结论:MTX抑制ADA表达,ADA表达下降使腺苷和脱氧腺苷表达升高,使MTX对破骨细胞的抑制作用减弱。Objective： To study the effects of adenosine deaminase and methotrexate （MTX） on osteoclastogenesis. Methods： The culture system of rat whole bone marrow cells （WBMCs） was utilized to evaluate osteoclastogenesis. Semi-quantitative RT-PCR was used to examine the expression of ADA mRNA in WBMCs culture system. Results： In the WBMCs culture system for evaluating oste- oclastogenesis, MTX-induced suppression of osteoclastogenesis was abrogated by adenosine or deoxyadenosine. The expression of ADA mRNA was markedly suppressed by MTX treatment. Conclusion： ADA decreased the MTX-induced suppression of osteoclastogenesis because MTX inhibits ADA expression followed by the increase of adenosine and deoxyadenosine.

关 键 词：破骨细胞 甲氨蝶呤 腺苷 脱氧腺苷 腺苷脱氨酶 

分 类 号：R782.6[医药卫生—口腔医学]