BMP2和BMP4及受体BMPR1A、BMPR2在口腔扁平苔藓患者外周血单个核细胞中的表达  

Expression of BMP2,BMP4,BMPR1A and BMPR2 in peripheral blood mononuclear cells of patients with oral lichen planus

作　　者：黎景景 王文梅[1,2,3] 朱雅男[1,2,3] 段宁[1,2,3] 蒋红柳[1,2,3] 白杨[1,2,3] 

机构地区：[1]南京大学医学院口腔医学院 [2]南京大学医学院附属口腔医院 [3]南京市口腔医院口腔黏膜病科,210008

出　　处：《实用口腔医学杂志》2014年第3期387-391,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81070839);江苏省医学领军人才与创新团队项目(编号:LJ201110);南京市医学科技发展重点项目(编号:ZKX10030)

摘　　要：目的:检测BMP2、BMP4及受体BMPR1A、BMPR2在口腔扁平苔藓(OLP)患者外周血单个核细胞(PBMCs)的表达。方法:用RT-PCR检测强的松治疗前后46名OLP患者和32名健康人PBMCs中BMP2、BMP4及BMPR1A、BMPR2的mRNA的表达水平。结果:OLP患者与对照组相比,BMP2 mRNA升高(P<0.05),BMPR2 mRNA降低(P<0.01),而BMP4及BMPR1A mRNA无明显差异(P>0.05)。网纹型OLP患者中BMP2 mRNA高于对照组(P<0.05),BMPR2 mRNA水平低于对照组(P<0.01);糜烂型OLP患者中BMP2 mRNA未发现改变,BMPR2 mRNA低于对照组(P<0.05)。强的松治疗后,OLP患者BMP2及BMPR1A mRNA升高(P<0.05),其中网纹型OLP患者BMPR1A升高(P<0.05),糜烂型OLP患者BMP2升高(P<0.05)。结论:OLP患者在PBMCs中BMP的异常表达,而强的松治疗对BMP表达有影响,且不同临床分型表达不同。Objective： To study the mRNA expression of BMP2, BMP4, BMPR1A and BMPR2 in the peripheral blood mononuclear cells of patients with oral lichen planus（OLP）. Methods： BMP2, BMP4, BMPR1A and BMPR2 mRNA in peripheral blood mononuclear cells isolated from 32 healthy controls and 46 OLP subjects before and after treatment with prednisone were detected by real-time RT-PCR. Results： Compared with controls, the expression level of BMP2 mRNA in OLP subjects was higher（ P 〈 0.05 ）, BMPR2 mRNA was lower（ P 〈0.01 ）. BMP2 mRNA in reticular OLP subjects was higher than that in the controls（ P 〈 0.05 ） and BMPR2 mRNA was lower（P 〈 0.01 ）. In all OLP patients prednisone treatment increased the mRNA expression of BMP2 and BMPR1A （P 〈 0.05 ）. In reticular OLP patients prednisone promoted BMPR1A mRNA expression（ P 〈 0.05 ）, in erosive OLP patients prednisone increased BMP2 expression （P 〈 0.05 ）. Conclusion： Predominant is effective in the treatment of OLP by regulation of BMP2, BMPR1A and BMPR2 expression.

关 键 词：口腔扁平苔藓 骨形成蛋白 外周血单个核细胞 强的松 

分 类 号：R781.5[医药卫生—口腔医学]