晚期糖基化终产物对人脂肪间充质干细胞功能影响的体外研究  

Effect of advanced glycosylation end products on function of human adipose tissue-derived stem cells in vitro

作　　者：王哲[1] 刘晓玉[2] 张殿宝[2] 王秋实[1] 

机构地区：[1]中国医科大学盛京医院输血科,辽宁沈阳110004 [2]中国医科大学细胞生物学卫生部重点实验室、干细胞与再生医学研究室,辽宁沈阳110001

出　　处：《中国病理生理杂志》2014年第5期897-901,共5页Chinese Journal of Pathophysiology

基　　金：辽宁省科技厅项目(No.2011225020;No.2012225014;No.2013225303)

摘　　要：目的:探讨晚期糖基化终产物(AGEs)对人脂肪来源的间充质干细胞(hADSCs)促进创伤修复功能的影响。方法:体外培养hADSCs,实验分为牛血清白蛋白(BSA)对照组、低浓度糖基化修饰的牛血清白蛋白(AGE-BSA)效应组和高浓度AGE-BSA效应组。采用WST法和Transwell迁移实验检测各组细胞增殖和迁移情况。应用实时定量PCR和ELISA检测各组细胞分泌血管内皮生长因子(VEGF)、肝细胞生长因子(HGF)和胰岛素样生长因子1(IGF-1)的表达。结果:与对照组相比,AGE-BSA组增殖能力和迁移能力明显下降(P<0.05),VEGF、HGF和IGF-1 mRNA及蛋白表达水平显著降低(P<0.05)。结论:AGEs能损伤hADSCs的促进创伤修复功能,因而能够影响hADSCs治疗糖尿病皮肤溃疡病的治疗效果。AIM： To explore the effect of advanced glycosylatinn end products （AGEs） on the function of human adipose-derived stem cells （hADSCs） in promoting wound healing. METHODS： hADSCs were isolated by conventional method in vitro anti divided into control bovine serum albumin （ BSA ） group, low-dose AGE-BSA group anti highdose AGE-BSA group. The proliferatiun and migration of hADSCs with different treatments were determined by WST-8 assay and Transwell assay, respectively. In addition, the expression of vascular endothelial growth factor （VEGF） , hepatoeyte grnwth factor （ HGF）, and insulin-like growth factor-1 （ IGF-1 ） at mRNA and protein levels was determined by realtime PCR and ELISA analysis. RESULTS： Compared with control group, the proliferation and migration abilities were significantly inhibited in the hADSCs of AGE-BSA group. The mRNA expression of VEGF, HGF and IGF-1 in AGE-BSA group was obviously lower than that in control group. The contents of VEGF, HGF and IGF-1 in hADSCs-c.onditioned medium in AGE-BSA group were also obviously lower than those in control group. CONCLUSION： AGEs alter the intrinsic properties of hADSCs and impair their functions in promoting wound healing, thus affecting the therapeutic potential of hADSCs in the treatment of diabetic ulcers.

关 键 词：晚期糖基化终产物 人脂肪间充质干细胞 细胞增殖 伤口愈合 

分 类 号：R363.21[医药卫生—病理学]