TNF-α及其Ⅰ型受体(P55)在翼状胬肉中的表达  

Expression of tumor necrosis factor-alpha and receptor I(P55) in pterygium

作　　者：吴兵 杨建 魏谨 马平 

机构地区：[1]江苏建康学院附属南京市高淳人民医院眼科,中国江苏省南京市211300

出　　处：《国际眼科杂志》2014年第6期1000-1004,共5页International Eye Science

摘　　要：目的:检测不同翼状胬肉组织中肿瘤坏死因子(tumor necrosis factor alpha,TNF-α)及其Ⅰ型受体P55的表达变化,探讨TNF-α及P55在翼状胬肉发病机制中的作用。方法:免疫组织化学方法(PV)检测翼状胬肉(72眼)及胬肉旁结膜组织(30眼)中TNF-α及其受体P55的表达,分析与翼状胬肉临床病理特征的关系。结果:TNF-α在翼状胬肉和胬肉旁结膜组织中的阳性表达率分别为65.3%(47/72),26.7%(8/30),差异有显著统计学意义(χ2=12.706,P<0.01)。P55在翼状胬肉和胬肉旁结膜组织中的阳性表达率分别为56.9%(41/72)及16.7%(5/30),差异有显著意义(χ2=13.875,P<0.01)。TNF-α在复发性胬肉的阳性表达率高于初发性胬肉(χ2=6.547,P=0.011),表达强度差异比较无统计学意义(F=1.288,P=0.393);进展期胬肉阳性表达率高于静止期胬肉(χ2=4.082,P=0.043),表达强度比较无统计学意义(F=0.489,P=0.708)。P55在复发性胬肉的阳性表达率高于初发性胬肉(χ2=9.907,P=0.002),表达强度比较无统计学意义(F=1.175,P=0.424);进展期胬肉的阳性表达率明显高于静止期胬肉(χ2=11.140,P=0.001),表达强度比较无统计学意义(F=0.665,P=0.621)。结论:TNF-α及P55在翼状胬肉中的阳性表达率随着发病状态和临床分期的进展而变化。TNF-α及P55表达与临床分类、临床分期、患者工作生存状况相关。TNF-α及P55在翼状胬肉不同发病状态及临床分期的表达强度无明显差异。AIM：To observe the expression of tumor necrosis factor- alpha（TNF-α）and its receptor I（P55）in different pterygium and discuss the role of TNF-α and receptor I（P55）in pterygium.METHODS： Immunohistochemistical staining method（PV）was adopted to detect the expression of TNF-α and receptor I in pterygium（72 eyes）and para-pterygium conjunctival tissue（30 eyes）. The relationship between the expression and clinical-pathological parameters was also analyzed. RESULTS： The positive rates of TNF-α were 65.3%（47/72）, 26.7%（8/30）in pterygium and para-pterygium conjunctival tissue. The positive expression of TNF-α had statistic difference between the two groups（χ2=12.706, P〈0.01）. The positive rates of TNF-α receptor I were 56.9%（41/72）, 16.7%（5/30）in pterygium and para-pterygium conjunctival tissue. The positive expression of P55 had statistic difference between the two groups（χ2=13.875, P〈0.01）. The positive rate of TNF-α in recurrent pterygium group was higher than primary pterygium group（χ2=6.547, P=0.011）. There had no statistically significance of the expression intensity between the two groups（F=1.288, P=0.393）; the positive rate in advanced pterygium group was higher than quiescent pterygium group（χ2=4.082, P=0.043）. The expression intensity had no statistically significance between the two groups（F=0.489, P=0.708）. The positive rate of P55 in recurrent pterygium group was higher than primary pterygium group（χ2=9.907, P=0.002）. There had no statistically significance of the two group＇s expression intensity（F=1.175, P=0.424）; the positive rate in advanced pterygium group was higher than in quiescent pterygium group（χ2=11.140, P=0.001）. The expression intensity had no statistically significance between the two groups（F=0.665, P=0.621）. CONCLUSION：The expression of TNF-α and P55 are changing according to the development of clinical staging and onset. The expression of TNF-α and P55 may be related to clinical classif

关 键 词：翼状胬肉 肿瘤坏死因子 肿瘤坏死因子受体 

分 类 号：R777.33[医药卫生—眼科]