ThD下调Hep-G2细胞骨桥蛋白的表达对其侵袭转移能力的影响研究  

Thalidomide Reduced the Expression of Osteopontin in Hep-G2 Cells and Effect of Its Invasion

作　　者：裴正浩[1] 林帆[1] 林坤鹏 覃金臣 伍兆锋[1] 黄炽明[2] 

机构地区：[1]广州医科大学附属广州市第一人民医院肝胆外科,广州510180 [2]广东省人民医院普通外科,广州516500

出　　处：《中国现代手术学杂志》2014年第2期90-95,共6页Chinese Journal of Modern Operative Surgery

摘　　要：目的了解ThD对体外肝癌细胞侵袭转移以及骨桥蛋白(OPN)表达的影响。方法应用细胞常规培养方法培养人肝癌细胞株Hep-G2,CCK-8法检测不同药物浓度及不同作用时间下ThD对肝癌细胞增殖的影响,细胞划痕试验验证ThD对肝癌细胞侵袭转移的能力的影响。用免疫细胞化学的方法检测用药组和对照组肝癌细胞中OPN的表达情况,Western blot法检测不同药物浓度及作用时间下肝癌细胞中OPN表达量。结果与对照组相比在作用24、48、72 h,ThD400μg/ml用药组对细胞生长均有抑制作用(P<0.01),并且抑制率和作用时间呈正相关。ThD用药组细胞迁移率在作用24、48、72 h后同对照组相比,P值均<0.05,差异有统计学意义。用药组同对照组免疫细胞化学染色积分为1.728±0.051、2.328±0.245,t值为-5.354,P<0.01。ThD用药组对细胞OPN表达有抑制作用,与对照组相比在作用24、48、72 h时,P值均<0.01,并且抑制率和作用时间呈正相关。结论 ThD对Hep-G2细胞的增殖、迁移及OPN的表达产生抑制作用,并且作用效果同药物浓度及作用时间呈正相关。Objective To investigate the effect of thalidomide on vitro liver cancer cell growth, inva- sion, metastasis and its osteopontin expression. Methods Human hepatoma cell line Hep-G2 was cultured by conventional culture methods. The cell proliferation of Hep-G2 by CCK-8 at different drug concentrations and times was assayed. Cells scratch test was used to verify the impact of thalidomide on the invasion and metastasis ability of Hep-G2. The level of osteopontin expression of Hep-G2 ceils was detected by the method of immunocytochemistry and Western blot after the action of different drugs and different reaction time. Result 400 μg/ml of thalidomide could inhibit cell proliferation, compared with the control group in 24, 48, 72 h （P 〈0.01 ）. And the effects positively correlated with reaction time. At the point of 24,48,72 h, compared with the control group,thalidomide significantly inhibited the cell migration（P 〈0.01 ）. The immunocytochemistry score was 1. 728 ± 0. 051 in ThD group and 2. 328 ± 0. 245 in control group （P 〈 0.01 ）. Thalidomide could inhibit the osteopontin expression（ P 〈 0.01 ） at 24,48,72 h time point, and the effects positively correlated with reaction time. Conclusions Thalidomide can inhibit the proliferation of hepatoma cells and the expression of OPN. And the effects positively correlate with the drug concentration and reaction time.

关 键 词：THD 肝肿瘤 骨桥蛋白 肿瘤浸润 肿瘤转移 

分 类 号：R735.7[医药卫生—肿瘤]