小分子化合物H-131抑制胃癌细胞增殖及机制  

The effect and mechanism of H-131 suppressing the proliferation of gastric cancer cells

作　　者：张健[1] 李瑞娟[2] 张红艳[1] 刘奇[1] 李丹妮[3] 李丰[1] 

机构地区：[1]中国医科大学基础医学院细胞生物教研室//教育部医学细胞生物学重点实验室,辽宁沈阳110001 [2]沈阳药科大学制药工程学院,辽宁沈阳110016 [3]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《解剖学研究》2014年第2期81-84,共4页Anatomy Research

基　　金：国家自然科学基金(81230077;81302127)

摘　　要：目的研究小分子化合物H-131对人胃癌细胞SGC7901、BGC823、MKN-45增殖的抑制作用及其相关机制。方法应用不同浓度的化合物H-131处理人胃癌细胞SGC7901、BGC823和MKN-45,采用MTT法检测该化合物对细胞增殖的影响,流式细胞术检测其对细胞周期的影响,Western blot检测化合物对周期调控蛋白Cyclin D1和CDK4表达的影响。结果化合物H-131能够以剂量依赖的方式抑制人胃癌细胞SGC7901、BGC823及MKN-45的增殖,并且能够抑制上述细胞G1期向S期的转化。Western blot结果显示H-131能够以剂量依赖的方式下调胃癌细胞SGC7901中Cyclin D1和CDK4的蛋白表达水平。结论化合物H-131能够通过下调胃癌细胞中Cyclin D1和CDK4的表达水平,抑制胃癌细胞G1期向S期的转化,进而抑制胃癌细胞增殖。Objective To investigate the effect and mechanism of H-131 on the proliferation of human gastric cancer cells. Methods MTY was used to observe the effect of H-131 on the proliferation of SGC7901, BGC823 and MKN-45 cells. Flow cytometry was used to check the effect of H-131 on cell cycle. Western blotting was adopted to identify the effect of H-131 on the expression of Cyclin D1 and CDK4. Results H-131 could suppress the proliferation of SGC7901, BGC823 and MKN-45 cells in a dose-dependent manner. It could also obviously inhibit the transition of cells from G1 to S phase. The Western blot re- suits showed that H-131 significantly down-regulated the expression levels of Cyclin D1 and CDK4 in SGC7901 cells. Conclu- sion H-131 suppresses Cyclin D1 and CDK4 expression, and inhibits the transition of cells from GI phase to S phase, and re- suits the anti-proliferative effect on human gastric cancer cells.

关 键 词：胃癌 H-131 细胞株 细胞周期蛋白D1 细胞周期蛋白依赖激酶 

分 类 号：R735.2[医药卫生—肿瘤]