MMP-3诱导人牙髓成纤维细胞CCN2mRNA的表达及意义  

Expression and Significance of CCN2 mRNA Induced by MMP-3 in Cultivate Human Pulp Fibroblasts

作　　者：王贺[1] 张娟[1] 尹硕[1] 李雯[1] 王晓龙[1] 唐宇欣[1] 张颖丽[1] 

机构地区：[1]吉林大学口腔医院牙体牙髓病科,吉林长春130021

出　　处：《口腔医学研究》2014年第5期399-402,共4页Journal of Oral Science Research

基　　金：吉林省科技发展计划(编号:20130102096JC);长春市科技计划项目(编号:12SF88)

摘　　要：目的:通过观察基质金属蛋白酶-3(MMP-3)对体外培养人牙髓成纤维细胞中结缔组织生长因子(CTGF/CCN2)mRNA的影响,揭示MMP-3促进牙髓损伤愈合的机制。方法:选取年轻患者因正畸或阻生拔除的健康第三磨牙,取出牙髓以常规体外酶消化培养法获得人牙髓成纤维细胞,利用RT-PCR方法检测经MMP-3诱导30min后人牙髓成纤维细胞中CCN2mRNA的表达。结果:正常组和实验组人牙髓成纤维细胞均有CCN2mRNA表达,但是经MMP-3诱导后的人牙髓成纤维细胞CCN2mRNA的表达更强。结论:MMP-3通过诱导CCN2mRNA的合成,从而促进牙髓损伤的愈合。Objective：To observe the effect of MMP-3on the mRNA of CCN2expression of cultivate human pulp fibroblasts in vitro and reveal the mechanism by which MMP-3promotes dental pulp wound healing.Methods：The human pulp fibroblasts were obtained from the healthy third molars which were extracted for orthodontic and impacted reason by tissue-explants collagenase digestion culture of pulp,The human pulp fibroblasts were stimulated by MMP-3.30minites later,the changes of CCN2mRNA expressed in human dental pulp fibroblasts were detected by means of RT-PCR.Results：CCN2mRNA expressed in both normal and experimental human pulp fibroblasts, but the expression of CCN2mRNA in human dental pulp fibroblasts which were induced by MMP-3was more significant.Conclusion：MMP-3which could induce the expression of CCN2mRNA in human pulp fibroblasts takes a critical part in dental pulp wound healing.

关 键 词：人牙髓成纤维细胞 基质金属蛋白酶-3 结缔组织生长因子 

分 类 号：R781.31[医药卫生—口腔医学]