乙醇消耗菌的分离鉴定及其对基因工程集胞藻乙醇产量的影响  

ISOLATION AND IDENTIFICATION OF ETHANOL CONSUMING MICROORGANISMS AND IMPACT ON ETHANOL PRODUCTION OF GENETICALLY ENGINEERED SYNECHOCYSTIS SP.

作　　者：葛平平[1,2] 陈林[1] 张维[1] 董庆喆 刘天中[1] 吕雪峰[1] 

机构地区：[1]中国科学院青岛生物能源与过程研究所生物燃料重点实验室,青岛266101 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2014年第3期487-494,共8页Acta Hydrobiologica Sinica

基　　金：国家高技术发展计划(863)(2012AA052103)资助

摘　　要：为研究产乙醇基因工程集胞藻培养液中的乙醇消耗菌污染情况及其对乙醇产量的影响,从污染的培养液中分离出4株乙醇消耗菌,通过16S rDNA、26S rDNA序列分析对分离出的菌株进行鉴定,并研究其乙醇消耗能力及对基因工程集胞藻乙醇产量的影响。结果表明,分离出的4株菌分别为红酵母(Rhodotorula sp.)、季也蒙酵母(Meyerozyma guilliermondii)、短波单胞菌(Brevundimonas sp.)、微杆菌(Microbacterium sp.)。其中乙醇消耗能力最强的是红酵母,乙醇比消耗速率达到391 g/(1015 cfu?d);其次为季也蒙酵母,乙醇比消耗速率为80.1 g/(1015 cfu?d);短波单胞菌和微杆菌的乙醇比消耗速率远低于红酵母和季也蒙酵母。将分离出的菌株与产乙醇集胞藻共培养7d后,污染红酵母、季也蒙酵母、短波单胞菌、微杆菌的实验组乙醇产量分别下降了53.8%、23.6%、40.7%、27.3%。4株菌对基因工程集胞藻的生长无明显影响,均通过直接消耗乙醇而降低集胞藻的乙醇产量。Four ethanol consuming microorganisms（GR13,GW13,GT17,and GY22） were isolated from the contaminated culture of genetically engineered Synechocystis sp.and were utilized to evaluate the effects of these microorganisms on the ethanol consuming capabilities and ethanol productions of Synechocystis sp.Based on the morphological characteristics and 16S rDNA and 26S rDNA gene sequence,the four strains were identified as Rhodotorula sp.,Meyerozyma sp.,Brevundimonas sp.,and Microbacterium sp.,respectively.We observed that strain GR13（Rhodotorula sp.） had the highest level of specific ethanol consumption rate [391 g/（1015 cfu？d）],followed by strain GW13（Meyerozyma sp.,[ 80.1 g/（1015 cfu？d）].While strains GT17（Brevundimonas sp.） and GY22（Microbacterium sp.） had much lower ethanol consumption rate.All four strains did not significantly affect the growth of Synechocystis sp.,however,they remarkably decreased the ethanol production of Synechocystis sp.The results of the co-culture experiment demonstrated that strains GR13,GW13,GT17,and GY22 diminished ethanol production of the genetically engineered Synechocystis sp.by 53.8%,23.6%,40.7% and 27.3%,respectively.

关 键 词：乙醇消耗菌 微生物污染 生物乙醇 基因工程集胞藻 

分 类 号：Q949.22[生物学—植物学]