2009年云南省 Echo30分离株 KM／A363／09全基因组序列分析  

Complete nucleotide sequence of a human echovirus 30 strain KM/A363/09 isolated in Yunnan, China in 2009

作　　者：白巍[1] 陈俊英[1] 潘玥[1] 朱艳菊[1] 邵聪文[1] 刘建生[1] 马绍辉[1] 

机构地区：[1]中国医学科学院 北京协和医学院 医学生物学研究所 云南省重大传染病疫苗研发重点实验室, 昆明650118

出　　处：《中华微生物学和免疫学杂志》2014年第5期388-392,共5页Chinese Journal of Microbiology and Immunology

基　　金：云南省应用基础研究项目（2013FZ136）

摘　　要：目的：对2009年从中国云南省1名脑炎患儿粪便标本中分离到的Echo30云南分离株KM／A363／09进行全基因组测序和分析，了解该株病毒的遗传特性。方法设计针对Echo30引物，提取病毒RNA、RT-PCR扩增和产物直接测序获得全基因组序列，利用Geneious和Mega5．1软件进行核苷酸、氨基酸序列比对及其系统进化分析，应用RDP3和SimPlot3．5．1重组软件对序列进行重组分析。结果该分离株全基因组核苷酸序列长度为7425 bp，编码2194个氨基酸。其核苷酸和氨基酸序列与其原型株Bastianni的同源性分别为81．2％和95．8％；与其他Echo30型毒株的各区段核苷酸和氨基酸同源性分别为81．2％～88．6％和95．8％～97．8％；进化分析发现KM／A363／09毒株属于中国Echo30分支中的一支。而且发现KM／A363／09株基因序列在非结构区可能存在重组事件。结论云南分离株KM／A363／09属于中国Echo30分支中的一支，而中国分离株已经发生一定的进化。Objective To analyze the genetic characteristics of the complete genome of a human echovirus 30 （Echo30） KM/A363/09 strain isolated in Yunnan, China in 2009.Methods Primers specif-ic for Echo30 were designed .The extracted RNA was amplified by using RT-PCR.Seven fragments covering the complete viral genome were sequenced and the complete sequences were aligned with other sequences of enterovirus reference strains downloaded from Genbank . By using Mega5.1, Geneious, RDP3 and SimPlot3.5.1 softwares, the phylogenetic and recombination analysis were carried out .Results The com-plete nucleotide sequence of KM/A363/09 isolate was 7425 bp in length, encoding 2194 amino acids.KM/A363/09 isolate was highly similar with Bastianni prototype strain showing the homology of 81.2%in nucle-otide and 95.8%in amino acid.The eight Echo30 isolates shared 81.2%-88.6%homologies in nucleotide sequences and 95.8%-97.8%in amino acid sequences .Phylogenetic analysis showed that the KM/A363/09 strain belonged to one clade of Echo 30 in China.The genetic recombination of KM/A363/09 isolate was detected in the non-structural region .Conclusion KM/A363/09 isolate belongs to one clade of Echo 30 in China indicating that the evolution of Echo 30 has occurred in China .

关 键 词：ECHO30 全基因序列 序列分析 ECHOVIRUS 30 

分 类 号：R440[医药卫生—诊断学]