冰片对Eca109/DDP细胞耐顺铂的逆转作用  

The reversal effect of borneol on cisplatin resistance in Eca109 /DDP cells

作　　者：戴翠萍[1] 张徐宁[1] 薛彩萍[1] 季宁东[1] 

机构地区：[1]淮阴卫生高等职业技术学校淮安市消化道肿瘤重点实验室,江苏淮安223300

出　　处：《广东医学》2014年第9期1317-1319,共3页Guangdong Medical Journal

基　　金：江苏省中医药科技项目(编号:LB09077);江苏省淮安市科技支撑计划(工业;社发)项目(编号:HAS2009002-2)

摘　　要：目的观察冰片对食管癌细胞Eca109/DDP耐顺铂(DDP)的逆转作用,并探讨其机制。方法 MTT法检测冰片单用及与DDP联用对Eca109/DDP细胞增殖的影响,观察冰片对Eca109/DDP细胞耐DDP的逆转作用,计算耐药逆转指数;Western blot法检测Eca109/DDP细胞P-糖蛋白(P-gp)的表达水平。结果 0.195、0.390、0.780、1.560μg/mL冰片对Eca109/DDP细胞无明显毒性作用(P>0.05),但与1μg/mL的DDP联用能浓度依赖性增加DDP对Eca109/DDP细胞的毒性(P<0.05),降低DDP对Eca109/DDP细胞的半数抑制浓度(IC50)(P<0.05),耐药逆转指数高达5.31,并能下调Eca109/DDP细胞P-gp的表达水平(P<0.05)。结论冰片能逆转Eca109/DDP细胞对DDP的耐药,其作用与下调Eca109/DDP细胞P-gp的表达有关。Objective To study the reversal effect and mechanism of borneol on cisplatin （ DDP） resistance in human esophageal carcinoma cell Eca 109/DDP.Methods The cytotoxic effect of borneol or DDP on Eca 109/DDP cells was measured by MTT and the drug -resistant reversal index （RI） was calculated.The expression of P-glycoprotein （P-gp） was detected by Western blot .Results MTT assay showed that borneol at the concentration no more than 1.560μg/mL had no obvious cytotoxicity to Eca 109/DDP cells （ P〉0.05 ） , but it could significantly enhance the cytotoxicity of DDP （1 μg/mL） to Eca109/DDP cells in a concentration -dependent manner （ P 〈0.05）, reduce IC50 of DDP on Eca109/DDP cells （P〈0.05）, and down-regulate the expression of P -gp of Eca109/DDP cells （P〈0.05）.RI of DDP was 5.31.Conclusion Borneol possesses a reversal effect on DDP resistance in Eca 109/DDP cells.The mechanism may be associated with the down -regulation of P-glycoprotein .

关 键 词：冰片 顺铂 ECA109 DDP 耐药逆转 P-糖蛋白 

分 类 号：R735.1[医药卫生—肿瘤]