丁苯酞对局灶性脑缺血大鼠脑水肿和梗死周围组织磷酸化肌球蛋白轻链表达的影响  

Effect of butylphthalide on brain edema and the expression of phosphorylated myosin light chain in peri-infarct tissue in focal cerebral ischemic rats

作　　者：王鹏[1] 王丽娜[2] 刘永亮[1] 李建民[3] 赵雅宁[1] 

机构地区：[1]河北联合大学,唐山063000 [2]河北联合大学附属唐山市人民医院,唐山063000 [3]河北联合大学附属医院神经外科,唐山063000

出　　处：《国际脑血管病杂志》2014年第5期376-380,共5页International Journal of Cerebrovascular Diseases

基　　金：河北省科技厅支撑计划（10276112D）;河北省自然科学基金（H2012401007）

摘　　要：目的 探讨丁苯酞对局灶性脑缺血大鼠脑水肿和梗死周围组织磷酸化肌球蛋白轻链表达的影响.方法 成年雄性Sprague-Daw ley大鼠212只,按随机数字表法分为假手术组（n=12）、脑缺血组（n=l00）和丁苯酞组（n=100）（40 mg/kg,1次/d,灌胃）;脑缺血组和丁苯酞组再分为6h、12 h、ld、3d和7d组（每个时间点各20只）.光化学法建立大鼠局灶性脑缺血模型,2,3,5-氯化三苯基四氮唑（2,3,5-triphenyltetrazolium chloride,TTC）染色法检测脑梗死体积（n=5）,干湿重法检测脑组织含水量（n=5）,免疫组化（n=5）和蛋白质印迹法（n=5）检测梗死周围皮质p-MLC蛋白表达.结果 TTC染色显示,假手术组未见梗死灶,丁苯酞组各时间点梗死体积均显著性小于脑缺血组相同时间点（P均＜0.05）.干湿重法显示,缺血组和丁苯酞组脑组织含水量均显著性高于假手术组（P均＜0.05）,但丁苯酞组各时间点脑组织含水量均显著性低于脑缺血组相同时间点（P均＜0.05）.免疫组化和蛋白质印迹均显示,缺血组和丁苯酞组脑组织梗死周围皮质p-MLC表达均较假手术组显著性上调（P均＜0.05）,但丁苯酞组各时间点脑组织梗死周围皮质p-MLC表达均显著性低于缺血组相同时间点（P均＜0.05）.结论 丁苯酞可通过减轻脑缺血引起的p-MLC表达上调而减轻脑水肿,进而缩小梗死体积,从而发挥神经保护作用.Objective To investigate the effect of butylphthalide on brain edema and the expression of phosphorylated myosin light chain in peri-infarct tissue in focal cerebral ischemic rats.Methods A total of 212 adult male Sprague-Dawley rats were divided into a sham operation group （n =12）,a cerebral ischemia group （n =100）,and a butylphthalide group （n =100） （40 mg/kg,1/day,gavage） according to the random number table.Both the cerebral ischemia group and the butylphthalide group were redivided into 6 h,12 h,1 d,3 d,and 7 d groups （n =20 for each time point）.A rat model of focal cerebral ischemic model was induced by photochemical method; 2,3,5-triphenyl tetrazolium chloride （TTC） staining was used to detect infarct volume （n =5）; wet-dry weight method was used to detect the water content in brain tissue （n =5）;immunohistochemistry （n =5） and Western blot （n =5） were used to detect the protein expression of periinfarct cortical p-MLC.Results TTC staining showed that no infarcts were observed in the sham operation group.The infarct volumes at each time points in the butylphthalide group were significantly less than those at the same time points in the cerebral ischemia group （all P 〈0.05）.Wet-dry weight method showed that the water contents in brain tissue in the cerebral ischemia group and the butylphthalide group were significantly higher than that in the sham operation group （all P 〈 0.05）,but the water contents in brain tissue at each time points in the butylphthalide group was significantly lower than those at the same time points in the cerebral ischemia group （all P 〈 0.05）.Both immunohistochemistry and Western blot showed that the expressions of peri-infarct cortical p-MLC in both the cerebral ischemia group and the butylphthalide group were upregulated significantly compared to the sham operation group （all P〈 0.05）,but the expressions of peri-infarct cortical p-MLC at each time points in the butylphthalide group was significantly lower than those at

关 键 词：脑缺血 肌球蛋白轻链 苯呋喃类 正丁基苯酞 脑水肿 血脑屏障 疾病模型 动物 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]