抗农作物病原真菌微生物的筛选及其聚酮合酶基因的分析  

Isolating of microorganism resistance to crop pathogenic fungi and its polyketone synthase gene analysis

作　　者：郭佳[1] 吴金龙[1] 唐颜苹[1] 谭晖[1] 赖树锦 李阳[1] 杨振飞[1] 陶新园 陈鑫[1] 李晓华[1] 

机构地区：[1]中南民族大学生命科学学院生物技术国家民委重点实验室,武汉430074

出　　处：《华中师范大学学报（自然科学版）》2014年第3期399-403,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(31070087;30570046);湖北省自然科学基金重点项目(2011CDA079);湖北省烟草公司面上项目(0710XYYC2012-01);国家大学生创新创业训练项目(GCX13104)

摘　　要：以5种农作物病原真菌为指示菌,从湖北省神农架地区的土壤中筛选得到1株抗真菌放线菌,命名为ⅡR21菌株.经过形态观察、培养特征、生理生化分析和16S rDNA序列同源性分析,初步鉴定该菌株属于链霉菌属.发酵液稳定性研究表明：ⅡR21菌株发酵液在-20℃～80℃范围内具有较强的稳定性;在pH 4.0～7.0范围内,ⅡR21菌株发酵液的抑菌活性比较稳定;紫外光照射对发酵液的抑菌活性没有影响.对放线菌ⅡR21菌株中Ⅰ型PKS基因族中KS基因进行PCR扩增和分析,与其他KS序列相似性比较低,与其系统分类最近的Streptom yces natalensis相似性达77％.Ⅱ R21 strain was isolated from the soil collected from Shenlongjia Hubei province with 5 pathogenic fungi of crop as indicator.Ⅱ R21 strain was classified as streptomyces by standard morphological,physiological properties and 16S rDNA alalysis.The experiments showed the antimicrobial substance in the fermentation broth of Ⅱ R21 strain was stability to temperature and UV-light.pH has a large impact on the antimicrobial activity strong of fermentation,but it was fairly stable at pH4.0 ～ 7.0.A polyketide synthase （PKS） gene was amplified by PCR using degenerated primers for the ketosynthase （KS） gene on the Ⅱ R21 strain.The phylogenetic tree and blast analysis revealed that sequence shared low similarity and 77％ closely identities to Streptomyces natalensis.

关 键 词：农作物病原真菌 链霉菌 16S RDNA Ⅰ型PKS基因族 KS基因 

分 类 号：Q939[生物学—微生物学]