NVP-BEZ235对卵巢癌细胞系SKOV3增殖及体内、体外迁移的影响  

Influence of NVP-BEZ235 on the inhibition of the human ovarian cancer cell line SKOV3 proliferation in vitro and the metastatic capacity in vitro/vivo

作　　者：秦宇[1] 赖慧玲[1] 赵雪娇[1] 王常玉[1] 

机构地区：[1]华中科技大学附属同济医院妇产科,武汉430030

出　　处：《实用药物与临床》2014年第5期521-524,共4页Practical Pharmacy and Clinical Remedies

基　　金：国家自然基金(G30973184)

摘　　要：目的探讨NVP-BEZ235对卵巢癌细胞SKOV3的增殖抑制及体内、体外转移的抑制作用。方法对贴壁生长的卵巢癌细胞SKOV3进行体外培养及传代,采用Cell Count Kit-8检测不同浓度NVP-BEZ235(125、250、500、1 000 nmol/mL)处理SKOV3细胞48 h后的增殖抑制效应。选取无增殖抑制的浓度125 nmol/mL处理细胞,Transwell法检测体外侵袭变化,斑马鱼移植癌细胞模型检测体内侵袭变化。结果 NVP-BEZ235能显著抑制卵巢癌细胞SKOV3体外增殖,Transwell法检测体外侵袭能力明显减弱,NVP-BEZ235显著抑制SKOV3细胞在斑马鱼体内转移。结论 NVP-BEZ235对于卵巢癌细胞SKOV3具有抑制增殖,减少体外体内侵袭,具有成为卵巢癌治疗药物的可能。Objective To investigate the inhibition effect of NVP-BEZ235 on proliferation in vitro and metastatic capacity of SKOV3 cell in vitro and in vivo. Methods Culture and passage the SKOV3 in vitro,assess the different doses（125,250,500,1 000 nmol /mL） NVP-BEZ235 inhibition effection on proliferation of SKOV3 by cell count Kit-8 after 48 hours. The metastatic capacity of SKOV3 were evaluated by transwell chamber assay in vitro and a zebrafish xenograft model in vivo. Results NVP-BEZ235 inhibited the proliferation of SKOV3 cancer cells. NVPBEZ235 inhibited the metastasis of SKOV3 in transwell chamber assay in vitro and Zebrafish xenograft in vivo. Conclusion NVP-BEZ235 can suppress the proliferation and invasiveness of ovarian cancer cells,and it might be a candidate treatment for ovarian cancer.

关 键 词：NVP-BEZ235 SKOV3细胞 增殖 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]