Siah2调控脯氨酰羟化酶3表达在非小细胞肺癌中的临床意义  

Clinical significance of regulation of prolyl hydroxylases 3 by Siah2 in non-small cell lung cancer

作　　者：潘坤[1] 李鹏[2] 张泽明[1] 赵学琴[1] 冯欣[1] 

机构地区：[1]河北大学附属医院呼吸内科,河北省保定市071000 [2]中国人民解放军第252医院心血管研究中心

出　　处：《河北医药》2014年第11期1608-1610,共3页Hebei Medical Journal

摘　　要：目的观察Siah2(seven in absentia2)及脯氨酰羟化酶3(prolyl hydroxylase 3,PHD3)在人非小细胞肺癌及癌旁组织中的表达,并探讨上述两者差异表达在非小细胞肺癌发病过程中的临床意义。方法应用RT-PCR检测非小细胞肺癌患者89例肿瘤及癌旁正常肺组织标本Siah2、PHD3 mRNA表达情况,Western blot检测上述指标的蛋白质表达水平,并分析其与肺癌临床特征的关系。结果非小细胞肺癌患者肿瘤组织中Siah2蛋白及mRNA表达量均明显高于癌旁组织(P<0.05),PHD3蛋白的表达在肿瘤组织中低于癌旁组织(P<0.05),PHD3 mRNA在肿瘤组织中的表达高于癌旁组织,Siah2蛋白与PHD3蛋白表达呈负相关(r=-0.735,P<0.05),Siah2蛋白与Siah2 mRNA表达呈正相关(r=0.821,P<0.05)。结论 Siah2可能通过调控PHD3表达水平在非小细胞肺癌发病中发挥作用。Objective To investigate the expression of Siah2（seven in absentia2） and prolyl hydroxylase 3（PHD3） in non-small cell lung cancer （ NSCLC） ,and to elucidate their clinical significance .Methods The mRNA expression levels of Siah2 and PHD3 were detected by RT-PCR in 89 cases of NSCLC and adjacent normal lung tissues , and their protein expression levels were detected by Western Blot .Results The expression levels of Siah 2 mRNA and protein in tumor tissues were significantly higher than those in adjacent tissues （ P 〈0.05）.The expression levels of PHD3 mRNA in tumor tissues were obviously higher than those in adjacent tissues , however , the expression levels of PHD 3 protein in tumor tissues were significantly lower than those in adjacent tissues （ P 〈0.05）.The expression of Siah2 protein was negatively correlated to that of PHD3 protein（ r =-0.735, P 〈0.05）,however,the expression of Siah2 protein was positively related with that of Siah 2 mRNA（ r =0.821, P 〈0.05）.Conclusion Siah2 may play a role in pathogenesis of NSCLC by regulating PHD 3 expression levels.

关 键 词：Siah2 脯氨酰羟化酶 低氧诱导因子-1Α 癌 非小细胞肺 

分 类 号：R734.2[医药卫生—肿瘤]