正交试验法在山葵多倍体诱导中的应用  

Application of Orthogonal Experiment in Polyploid Induction of Wasabi

作　　者：王跃华[1] 段茂华[1] 任三军 陈传凤 彭双[1] 严幸林 孙燕霞[1] 

机构地区：[1]成都大学生物产业学院,四川成都610016 [2]广元市玺府开发有限公司,四川广元648017

出　　处：《种子》2014年第5期42-45,共4页Seed

基　　金：广元市科技支撑计划项目(2012)

摘　　要：目的:改良山葵现有品种,丰富物种资源,增加其有效成分的含量。方法:采用正交试验法考察了秋水仙素浓度、秋水仙素浸泡时间和浸泡温度对山葵种子多倍体诱导率的影响。结果:在考察的各因素中,对山葵植物种子多倍体诱导率的影响大小为秋水仙素的浓度>秋水仙素浸泡时间>秋水仙素浸泡的温度。结论:用L9(33)正交试验筛选出诱导山葵多倍体植株的最佳组合条件为A3 B3 C3,即用1.0%的秋水仙素溶液在20℃的条件下浸泡山葵种子24 h,其多倍体诱导率最高,为50.847%。经植物形态组织学和染色体数检测,诱导后的植物材料具有明显的多倍体特征。Objective ： Improved Wasabi existing varieties, enriched in species resources, increased their content of active ingredients. Method：Investigated by orthogonal test concentration of colchicine, colchicine different immersion time and temperature on the induction of polyploidy horseradish seeds. Result：The factors in the study of polyploid plants wasabi induction rate the degree of influence is ： colchicine concentrations 〉 colchicine immersion time 〉 Temperature. Conclusion ： Using L9 （ 33 ） orthogonal test induced polyploid plants wasabi optimal induction conditions, the best combination of polyploid induction A 3 B 3 C 3, which uses 1.0% colchicine treatment of seeds, placed at 20℃ 24 h, its highest rate of polyploid induction up to 50. 847%. Plant material has obvious characteristics after induction of polyploid by detection of histology plant morphology and chromosome number.

关 键 词：正交试验 山葵 秋水仙素 

分 类 号：S567[农业科学—中草药栽培]