高灵敏荧光法测定盐酸雷尼替丁  

Highly sensitive fluorescence method for the determination of ranitidine hydrochloride

作　　者：刘奇[1] 王静 徐艳茹[1] 叶存玲[1] 李伟[1] 

机构地区：[1]河南师范大学化学化工学院,化学制药及生物医用材料河南省工程实验室,新乡453007

出　　处：《分析试验室》2014年第6期656-658,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21107022)资助

摘　　要：基于N-溴代丁二酰亚胺可与盐酸雷尼替丁发生氧化还原反应,剩余的N-溴代丁二酰亚胺能氧化亚甲基蓝,β-环糊精对未参加反应的亚甲基蓝具有荧光增强作用,可使其荧光增强近2倍,通过测定亚甲基蓝的荧光强度间接测定盐酸雷尼替丁的含量,建立了测定盐酸雷尼替丁荧光新方法,体系的最大激发波长为647 nm,最大发射波长为683 nm,线性范围为0.05～1.55 mg/L,检出限为7μg/L,相对标准偏差为0.2%。方法已用于实际样品的测定。A new, accurate and sensitive fluorescence method is proposed for the determination of ranitidine hydrochloride （RHCI） in pharmaceutical formulation. The method is based on the enhancement of RHCl on the fluorescence intensity of the product yielded in the redox reaction between NBS and MB in hydrochloric acidic medium, in which β-CD acts as a sensitizing effect reagent. The fluorescence intensity of the complex is also enhanced greatly. Through an indirect way, RHCL can be determined by measuring the fluorescence intensity of the MB. Its maximum excitation wavelength is at 647 nm, and the maximum emission wavelength is at 683 nm. The linear range detection limit and relative standard deviation are 0. 05 - 1.55mg/L, 7 μg/L and 0. 20% , respectively. The method was successfully applied to the assay of RHCI in capsule forms and the results were consistent with those of the reference method.

关 键 词：荧光增敏法 Β-环糊精 亚甲基蓝 盐酸雷尼替丁 N-溴代丁二酰亚胺 

分 类 号：O657.3[理学—分析化学]