复方丹参片对阿尔茨海默病转基因细胞模型Tau蛋白表达的影响  

The effect of Compound Danshen Tablets on the Expression of Tau in AD Transgenic Cell Model

作　　者：刘利娟[1] 周德生[1] 肖志杰 陈瑶[1] 胡华[1] 孙晓鹏[1] 覃仁安[3] 

机构地区：[1]湖南中医药大学第一附属医院神经内科,湖南长沙410007 [2]湖南湘雅医学院第二附属医院神经内科,湖南长沙410011 [3]广州白云山和记黄埔中药有限公司现代中药研究院,广东广州510515

出　　处：《湖南中医药大学学报》2014年第5期14-17,共4页Journal of Hunan University of Chinese Medicine

基　　金："重大新药创制"国家科技重大专项资助项目(2011X09201-201-01);湖南省自然科学基金资助项目(14JJ6038);湖南省科学技术厅科技计划资助项目(2014SK3037)

摘　　要：目的研究复方丹参片对阿尔茨海默病(Alzheimer disease,AD)转基因细胞模型微管相关蛋白(Tau蛋白)表达的影响。方法将SD大鼠随机分成复方丹参片0.5、1、2、4倍剂量组、空白对照组及多奈哌齐组,分别灌服相应药液,空白组给予等容积生理盐水,1次/日,连续7 d之后,取各组血清培养AD转基因细胞,于24、48、72 h采用Western Blot法分析AD转基因细胞Tau蛋白表达情况。结果与空白对照组相比,24 h时复方丹参片1、2、4倍剂量组能降低Tau蛋白表达,差异有统计学意义(P<0.01);48 h时复方丹参片2、4倍剂量组差异有统计学意义(P<0.01);72 h时各剂量组间差异均无统计学意义(P>0.05)。结论复方丹参片2倍剂量48 h时能显著减少AD转基因细胞模型Tau蛋白表达。Objective Study on the expression of the Tau that ADtransgenic cell has been cultured by Danshen Tablets （DT）. Methods SD rats were randomly divided into 6 groups, including 0.5 dose, 1.0 dose, 2.0 dose, 4.0 dose DT groups, blank control group and donepezil group. Each group rats respectively were given different concentrations of drugs, blank group was given equal normal saline, 1 time a day, 7 days in a row . Then each containing medicine serum of rats were taken to cultivate the AD transgenic cells, and using the m ethods that Western blot to test the levels of Tau protein after cultured 24h, 48h, 72h. Results Compared with the blank control group, At 24h time point, the expression of Tau were declined by 1×, dose of DT , there were significant effects （P0.05）. Conclusion 2 × dose of DCT could reduce the level of Tau obviously at 48h on the AD transgenic cell model.

关 键 词：复方丹参片 TAU蛋白 阿尔茨海默病 转基因细胞模型 

分 类 号：R741[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]