电针曲池与上巨虚穴对溃疡性结肠炎大鼠NF-κB及HMGB1表达的影响  

The Effect of Quchi and Shangjuxu Acupoints on the Level of NF-κB, HMGB1 in Ulcerative Colitis Rats Models via Electro-acupuncture

作　　者：杨璐佳 张泓[1] 张雨辰[2] 邓石峰 王珍珍[1] 粟艳梅[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《湖南中医药大学学报》2014年第5期58-61,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81173327/H2718);湖南省教育厅创新平台项目(11K047)

摘　　要：目的观察电针溃疡性结肠炎(ulcerative colitis,UC)大鼠大肠经经合穴"曲池"、下合穴"上巨虚"后对核因子-κB((nuclearfactorκB,NF-κB)、高迁移率族蛋白1(high mobility group box 1,HMGB1)表达的影响。方法以2-4-6三硝基苯磺酸(TNBS)/乙醇溶液灌肠诱导建立SD大鼠UC模型,曲池组与上巨虚组造模成功后分别电针曲池穴和上巨虚穴,连续治疗10 d,第11天处死所有大鼠,肉眼观察大鼠结肠溃疡评分,提取结肠组织检测其NF-κB、HMGB1的表达。结果造模成功后,模型组大鼠结肠肉眼溃疡评分、结肠组织NF-κB、HMGB1表达均明显增高(P<0.01);电针治疗后,上巨虚组降低溃疡评分(P<0.01),曲池组、上巨虚组均能降低NF-κB、HMGB1在结肠中的表达(P<0.01);与曲池组相比,上巨虚组溃疡评分及NF-κB、HMGB1表达均明显降低(P<0.01)。结论电针对UC的治疗作用可能是通过调节NF-κB、HMGB1等来实现的;电针上巨虚的治疗作用优于曲池,说明"上巨虚"存在相对特异性,部分证实"合治内府"中的"合"主要应指下合穴,其具体内涵仍需进一步研究。Objective To observe the expression level of high m obility group box 1 （HMGB1）, nuclearfactorκB （N F-κB） in the ulcerative colitis（UC ） rats and study related m echanism to further understand the m eaning of＂He-sea points＂ in ＂curing viscera diseases by He-sea points＂ after electro-acupuncturing ＂Quchi＂ and ＂Shangjuxu＂ acupoints. Methods The UC rats m odels were given trinitrobenzene sulphoneacid （TN BS）/ethanol enem a, then ＂Quchi＂ group and ＂Shangjuxu＂ group respec-tively received electro-acupuncture at＂Quchi＂ and ＂Shangjuxu＂ acupoints for 10 consecutive days. 24h after the last treatm ent, we dissected all rats and collected colon tissue to observe ulcer score and the expres-sion level of HMGB1 and N F-κB. R esults ① Compared with control group, thulcer score,HMGB1 and NF-κB signifcantly increased （P〈0.01） in m odel group.② Compared with model group,＂Quchi＂ group and ＂Shangjuxu＂ group made obvious decrease in the ulcer score,HMGB1 and NF-κB （P〈0.01）.③ Com-pared with ＂Quchi＂ group, the ulcer score,HMGB1 and NF-κB decreased significantly（P〈0.01）. Conclusion ① Both of the large intestinal meridian＇s lower He-sea point ＂Shangjuxu＂ and upper He-sea point＂Quchi＂ can improve the UC through decreasing the ulcer score , HMGB1 and N F-κB of the ulcera-tive colitis. ② The treatment effect of ＂Shangjuxu＂ on the UC is better than ＂Quchi＂, it shows that＂Shangjuxu＂ possess relative specificity.③ Our results im partially suggest that＂He points＂ in ＂curing vis-cera diseases by He points＂ mainly implicates the lower He point, and the specific mechanism still needs further research.

关 键 词：溃疡性结肠炎 电针 核因子-κB 高迁移率族蛋白1 大鼠 曲池 上巨虚 

分 类 号：R574.62[医药卫生—消化系统] R246.1[医药卫生—内科学]