新型甘蓝型油菜CMS选育及分子鉴定  

Breeding and molecular identification of new cytoplasmic male sterile(CMS) in Brassica napus L.

作　　者：姚祥坦[1] 张芳[2] 王润屹 徐素琴[1] 董衡[2] 黄鹂[2] 

机构地区：[1]浙江省嘉兴市农业科学研究院,嘉兴314016 [2]浙江大学蔬菜研究所,杭州310058

出　　处：《安徽农业大学学报》2014年第3期406-410,共5页Journal of Anhui Agricultural University

基　　金：嘉兴市科技计划项目(2010AZ2016;2012AZ2020);浙江省科技厅项目(2012C12902-1-4)共同资助

摘　　要：以榨菜细胞质雄性不育系(CMS)为不育胞质来源,甘蓝型油菜自交系为父本,通过连续回交定向选育方法进行榨菜胞质甘蓝型油菜CMS的转育,获得了原始不育系‘93-12A’,并通过父本载体的转换对其进行改良,获得了一份综合性状较优良的不育系‘嘉油58A’;根据已报道的油菜CMS相关基因orf224、orf138和叶用芥菜CMS相关基因orf220设计特异引物,对榨菜胞质甘蓝型油菜CMS及其保持系的基因组DNA进行PCR扩增,结果显示,仅orf220的特异引物能在原始不育系‘93-12A’和改良不育系‘嘉油58A’中扩增出特异条带,扩增产物序列与orf220具有100%的同源性,从而从分子水平验证‘93-12A’和‘嘉油58A’的不育胞质来源于榨菜。A new Brassica napus cytoplasmic male sterile （CMS） line, ＇93-12A＇ was developed through cross and continuous backcross between tuber mustard CMS line and the Brassica napus variety ＇92-58＇. By backcrossing it to the inbred lines ＇022058＇, we finally got a stable CMS line named ＇Jiayou58A＇ with im- proved comprehensive agronomic characters. To identify the origin of cytoplasmic male sterility of ＇93-12A＇ and ＇Jiayou58A＇, the specific primers were amplified according to the reported fertility-related gene orf224, orf138 of B. napus and orf220 of B. juncea in its genome. The results showed that only the specific band of orf220 was detected in the new CMS line ＇93-12A＇ and the improved CMS line ＇Jiayou58A＇. Sequencing of the bands confirmed that the male sterility cytoplasm of ＇93-12A＇ and ＇Jiayou58A＇ is originated from the tuber mustard （B. juncea）.

关 键 词：甘蓝型油菜 榨菜 细胞质雄性不育 回交 分子鉴定 

分 类 号：S565.4[农业科学—作物学]