去铁疗法在实验性肝损伤中的保护作用  

Protective effect of phlebotomy in experimental liver damage

作　　者：白玉[1] 曹治宸[1] 庄甲举[2] 勾凌燕[2] 刘景冬[2] 宋艳改[1] 

机构地区：[1]河北医科大学第三医院感染科,河北省石家庄市050051 [2]白求恩军医学院仪器中心,河北省石家庄市050081

出　　处：《世界华人消化杂志》2001年第2期149-152,共4页World Chinese Journal of Digestology

摘　　要：目的探讨去铁疗法在实验性肝损伤中的保护作用机制。方法 Wistar大鼠56只,随机分为6组:空白组、高铁组、低铁组、CCl_4组、CCl_4+高铁组和CCl_4+低铁组,复制高组铁模型、铁模型和CCl_4攻击肝损伤模型。观察各组血清铁(SI)、ALT、AST、丙二醛(MDA)、肝组织铁含量(HIC)以及病理变化。结果低铁组与空白组、CCl_4+低铁组与CCl_4+铁组比较,HIC明显降低(q_1=17.79,P<0.01;q_2=17.56,P<0.01)。CCl_4+低铁组与CCl_4+高铁组比较,ALT,MDA显著性降低(q_1=3.96,P<0.05;q_2=6.70,P<0.01)。铁在肝脏中的分布有三种类型:窦周型、血管周围型、肝细胞型,无肝损伤或轻微肝损伤时,以窦周型为主。肝损伤严重时,以血管周围型为主,并且病变周围,铁沉积增多。结论放血疗法可以减轻高铁负荷加重的实验性大鼠肝损伤。AIM To investigate the protective function of phlebotomy in liver damage aggravated by iron overload. METHODS Fifty-six male Wistar rats were divided into six groups randomly: blank group, iron overload (IO) group, phlebotomy (P) group, CCI_4 group, CCI_4 + IO group and CCI_4 + P group. The models were made with iron dextran (ID), CCI_4 and phlebotomy, respectively. The following parameters were examined: serum iron (SI), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, malondialdehyde (MDA) and hepatic iron concentration (HIC) in liver. The distribution of iron and pathological changes in liver were also observed. RESULTS HIC decreased more significantly in P group than blank group and in CCI_4 + P group than CCI_4 + IO group (q_1 = 17.79, P<0.01; q_2=17.56,P<0.01). ALT and MDA were significantly lower in CCI_4 + P group than in CCI_4+IO group (q_1 = 3.96, P<0.05; q_2 = 6.70, P<0.01). Pathological study revealed that blue iron particles deposited with three forms: hepatic sinus, hepatocytes or vessel zones. The more the iron particles deposited, the more serious the necrosis zone was. CONCLUSION Phlebotomy may alleviate iron overload to protect hepatocytes from damage.

关 键 词：肝损伤 丙氨酸转氨酶 天冬氨酸转氨酶 去铁疗法 治疗 

分 类 号：R575[医药卫生—消化系统]