杜仲雌雄株叶高效液相色谱指纹图谱和次生代谢产物的含量比较  

HPLC fingerprint and several secondary metabolites content in Eucommia ulmoides leaf

作　　者：叶东旭[1,2] 李钦[1,2] 杜红岩[3] 杜兰英[3] 付建敏[3] 

机构地区：[1]河南大学药学院,河南开封475004 [2]河南大学中药研究所,河南开封475004 [3]中国林业科学院经济林研究开发中心,河南郑州450003

出　　处：《时珍国医国药》2014年第5期1077-1079,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家林业公益性行业科研专项(No.201004029);河南省科技攻关重点项目(No.102102310188);河南省教育厅自然科学基金(No.2008A360003)

摘　　要：目的采用反相高效液相色谱法,建立杜仲叶的HPLC指纹图谱,比较杜仲雄株叶和雌株叶中几种次生代谢产物的含量。方法采用Ameritech C18(250 mm×4.6 mm,5μm)色谱柱,甲醇-0.05%磷酸溶液梯度洗脱;体积流量1.0 ml/min;检测波长238 nm,柱温25℃。结果对同一日期采集的杜仲叶指纹图谱进行评价,生成对照图谱,确定了19个共有峰。杜仲雄株叶中绿原酸含量略高于杜仲雌株叶,杜仲雄株叶中京尼平苷酸含量和京尼平苷含量略低于杜仲雌株叶,但差异不明显。结论性别对杜仲叶中几种次生代谢产物的含量无明显影响。Objective Use reversed - phase of HPLC to establish HPLC fingerprint of Eucommia ulmoides leaf and compare the content of several secondary metabolites in Eucommia ulmoides male and female leaves. Methods RP - HPLC method was used for the determination. The separation was performed on an Ameritech C18 column （250 mm×4.6 mm,5 μm）, the mobile phase was methanol with 0.05% phosphoric acid in the gradient elution mode. The flow rate was 1.0mL/min. The detective wavelength was set at 238nm. The temperature of column was maintained at 259C. Results The fingerprint of samples from same picking time and same place were used to get the reference chromatogram composed of nineteen common peaks. The chlorogenic acid content in Eucommia ulmoides male leaf was slightly higher than Ettcommia ulmoides female leaves ; the geniposidic acid and geniposide contents were slightly lower than Eucommia female leaves, but the difference is not obvious. Conclusion the gender has no significant effect on the content of several secondary metabolites in Eucommia ulmoides leaves.

关 键 词：杜仲叶 高效液相色谱法 指纹图谱 次生代谢产物 

分 类 号：R284.2[医药卫生—中药学]