绿海龟α-actin基因的cDNA克隆与序列分析  

Cloning and sequence analysis of full-length cDNA of α-actin gene from Chelonia mydas

作　　者：陶翠花[1] 刘莹莹[2] 赵丽媛[1] 许敏[1,2] 祝茜[1,2] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]山东大学(威海)海洋学院,山东威海264209

出　　处：《海洋科学》2014年第3期98-103,共6页Marine Sciences

基　　金：国家海洋局第三海洋研究所基本科研业务费专项资金项目(2010006);国家海洋公益性行业科研专项(201105011)

摘　　要：为探究绿海龟（Chelonia mydas）α-actin基因序列的相关信息,作者利用RT-PCR和RACE方法从绿海龟肌肉组织中获得了α-actin基因的cDNA全长序列,共1347bp（GenBank登录号为JX073650）。所得序列包含一个1134 bp的开放阅读框,编码由377个氨基酸组成的蛋白,该蛋白7~377位为Actin结构域,14~17位有一个糖基化位点,无信号肽;预测分子量为42.0 kDa,理论等电点为5.23。将编码区序列与GenBank上同源序列进行比对发现,核苷酸序列相似性均在85.4%以上,氨基酸序列相似性均在98.9%以上,说明α-actin基因作为编码蛋白是高度保守的。To explore the sequence and characteristic of α-actin gene from Chelonia mydas, the full-length cDNA sequence of α-actin gene was cloned using RT-PCR and RACE technique, which was consisted of 1347 bp nucleotides（GenBank accession number： JX073650）, with a putative open reading frame（ORF） of 1134 bp encoding a deduced 377 amino acid protein containing a glycosylation site（from 14 to 17） and an Actin domain（from 7 to 377）. The molecular weight of the protein was 42.0 kDa and the isoelectric point（pI） was 5.23. The nucleotide sequence similarity of α-actin gene between C. mydas and other species was above 85.4%, while the similarity of amino acid sequence was more than 98.9%, suggesting that α-actin gene was highly conserved. This study has enriched the Actin gene database and provided basic data for further studies on expression and function of relevant genes.

关 键 词：绿海龟(Chelonia mydas) α-actin基因 RACE技术 

分 类 号：Q781[生物学—分子生物学]