3个绵羊群体MSTN基因编码区PCR-SSCP分析  

PCR-SSCP Analysis on MSTN Gene in the Coding Region of Three Sheep Breeds

作　　者：任有蛇[1] 白元生[2] 杨子森[2] 师周戈[2] 刘晓妮[2] 张春香[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]山西省农业厅生态畜牧产业管理站

出　　处：《中国草食动物科学》2014年第3期5-8,共4页China Herbivore Science

基　　金：山西省肉羊联合育种科技合作项目

摘　　要：采用PCR-SSCP技术分析了3个不同绵羊品种(杜泊绵羊、小尾寒羊、晋中绵羊)肌肉生长抑制素(MSTN)基因的单核苷酸多态性。根据3个绵羊品种MSTN基因Exon1(P1)、Exon2(P2和P3)和Exon3(P4和P5)序列设计5对PCR-SSCP引物并扩增,经SSCP分析,P1位点检测到A和B两个等位基因,AA、AB、BB三种基因型;且在晋中绵羊上处于Hardy-Weinberg平衡状态,而在小尾寒羊和杜泊绵羊上极显著偏离Hardy-Weinberg平衡状态。P2和P5位点没有检测到多态位点。P3位点检测到A和B两个等位基因,AA、BB两种基因型;且在3个绵羊品种上极显著偏离HardyWeinberg平衡状态。P4位点检测到A、B和C三个等位基因,AA、AB、AC三种基因型;且在小尾寒羊上处于Hardy-Weinberg平衡状态,在晋中绵羊上处于Hardy-Weinberg不平衡状态,在杜泊绵羊上极显著偏离Hardy-Weinberg平衡状态。The single nucleotide polymorphisms of myostatin （MSTN）gene in the three sheep breeds （Dorper sheep,Small-tailed Han Sheep and Jinzhong sheep）were analysed in this paper.According to the sequences of Exon1 （P1）,Exon2（P2 and P3）,Exon3 （P4 and P5）ofMSTN gene in the sheeps,5 pairs of PCR-SSCP primers were designed and amplified by PCR-SSCP,The results showed that there were 2 alleles and AA,AB and BB genotypes in P1.the P1 loci in Jinzhong sheep were consistent with the Hardy-Weinberg equilibrium and in the Small Tailed Han sheep and Dorper sheep were significantly deviated from it （P ＜ 0.01）.Polymorphism was not detected in P2 and P5.There were 2 alleles and AA and AB genotypes in P3,and three sheep breeds were significantly deviated from the Hardy-Weinberg equilibrium （P ＜ 0.01）.There were 3 alleles and AA,AB,and AC genotypes in P4.the P4 loci in the Small Tailed Han sheep were consistent with the Hardy-Weinberg equilibrium,in Jinzhong sheep not,and in Dorper sheep significantly deviated from it （P ＜ 0.01）.

关 键 词：MSTN PCR-SSCP 等位基因 多态性 

分 类 号：S826.2[农业科学—畜牧学]