羟基喜树碱脂质体与顺铂联用对耐顺铂的人食管癌Eca109细胞周期和凋亡的影响  

Effects of hydroxycamptothecin liposome combined with cisplatin on cell cycle and apoptosis of cisplatin-resistanted human esophageal carcinoma Eca109 cell

作　　者：戴翠萍[1] 张徐宁[1] 薛彩萍[1] 刘家秀[1] 张玉领[1] 杨学艺[1] 陈文中 季宁东[1] 

机构地区：[1]淮阴卫生高等职业技术学校淮安市消化道肿瘤重点实验室,淮安223300 [2]南京思科药业有限公司,南京210061

出　　处：《郑州大学学报（医学版）》2014年第3期301-304,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：江苏省卫生职业教育研究指导性项目JZ200911;淮安市科技支撑计划项目HAS2009002-5

摘　　要：目的:研究羟基喜树碱脂质体(LHCPT)与顺铂(DDP)联用对耐DDP的人食管癌细胞Eca109细胞(Eca109/DDP)细胞周期和凋亡的影响。方法:取对数生长期的Eca109/DDP细胞,分别用0、1 mg/L DDP处理,再用0.00、0.49、0.98、1.96、3.92、7.84μg/L LHCPT处理72 h,MTT法测定细胞增殖状况。取对数生长期的Eca109/DDP细胞,分别用0、1 mg/L DDP处理后,再用0.00、3.92μg/L LHCPT处理72 h,用流式细胞仪检测细胞周期分布及细胞凋亡情况。结果:相对于单独应用,LHCPT与DDP联合应用抑制了Eca109/DDP细胞的增殖(FLHCPT=40.330,FDDP=641.010,F交互=20.330,P均<0.001),以3.92μg/L LHCPT与1 mg/L DDP联用效果最佳(P<0.05)。相对于单独应用,3.92μg/L LHCPT与1 mg/L DDP联用可阻滞Eca109/DDP细胞在G0/G1期(FLHCPT=16.184,FDDP=33.660,F交互=15.100,P均<0.05),同时增加细胞的早期凋亡率、晚期凋亡率和总凋亡率(P均<0.05),二者具有协同效应。结论:LHCPT具有辅助DDP治疗食管癌的作用。Aim： To investigate the effects of hydroxycamptothecin liposome（LHCPT） combined with cisplatin（DDP） on cell cycle and apoptosis of DDP-resistanted human esophageal carcinoma cell Ecal09 （Ecal09/DDP）. Methods： EcalOg/DDP cells were treated with 0,1 mg/L DDP, and then treated with 0. 00 ,0. 49 ,0. 98 ,1. 96 ,3. 92 ,7. 84 μg/L LHCPT for 72 h, respectively; MTT method was applicated to detect the cell proliferation. Ecal09/DDP cells were treated with 0,1 mg/L DDP,and then treated with 0.00,3.92 μg/L LHCPT for 72 h, respectively;flow cytometry was used to study the cell cycle and apoptosis. Results：Compared with single drug treatment, the growth inhibition effect on Ecal09/DDP cells in LHCPT ＋ DDP group was significant（ FLHCPT = 40. 330, FDDp -= 641. 010, Fi ion = 20. 330, P 〈 0. 001 ） , especially 3.92 p.g/L LHCPT ＋ 1 mg/L DDP. Compared with single drug treatment, 3.92 μg/L LHCPT ＋ 1 mg/L DDP could significantly block Eca109/DDP ceils at G0/G1 phase（FLncpT = 16. 184,FDDP =33. 660,Finteraction = 15. 100,P 〈0.05）, and increase the early apoptotic rate,late apoptotic rate and total apoptotic rate（P 〈 0.05）. Conclusion： LHCPT has the potential to be an adjunct to chemotherapy for esophageal carcinoma.

关 键 词：羟基喜树碱脂质体 顺铂 食管癌 细胞周期 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]