肺癌患者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的表达  

Expressions of DNMT1,DNMT3a,DNMT3b and HDAC1 in serum from patients with lung cancer

作　　者：冯悦静[1] 魏小玲[2] 尤爱国[3] 周安燕[1] 吴拥军[2] 王威[2] 何其栋[2] 王静[1] 

机构地区：[1]郑州大学第一附属医院呼吸内科,郑州450052 [2]郑州大学公共卫生学院毒理学教研室,郑州4500013 [3]河南省疾病预防控制中心传染病预防控制所,郑州450016

出　　处：《郑州大学学报（医学版）》2014年第3期323-326,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30972457;河南省重大科技攻关项目112102310102

摘　　要：目的:研究肺癌患者血清中DNA甲基化转移酶(DNMT)1、DNMT3a、DNMT3b和组蛋白去乙酰化酶1(HDAC1)蛋白的表达情况。方法:采用酶联免疫吸附试验(ELISA)方法测定136例肺癌患者、140例肺良性疾病及145例健康体检者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的表达,并分析DNMT1、DNMT3a、DNMT3b和HDAC1蛋白表达与肺癌临床病理特征的关系。结果:3组血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白表达差异有统计学意义(F=3.281、62.510、37.273、57.721,P<0.05),肺癌患者均高于正常对照及肺良性疾病患者;非条件logistic回归分析提示DNMT1、DNMT3a、DNMT3b和HDAC1蛋白高表达均可影响肺癌的发生发展(P<0.001);肺癌患者血清中DNMT1、DNMT 3a、DNMT 3b和HDAC1蛋白表达无组织学类型特异性(P>0.05),但不同临床分期其蛋白表达差异有统计学意义(t=0.843、0.244、0.222、0.878,P<0.05)。结论:肺癌患者血清中DNMT1、DNMT3a、DNMT3b和HDAC1蛋白的高表达可能在肺癌的发生发展中发挥一定的作用。Aim：To study the expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in serum from patients with lung cancer.Methods：ELISA was used to measure the expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in the serum from 136 lung cancer patients ,140 cases of lung benign diseases and 145 healthy people （ control group ） , and the relationship between the expressions of DNMT 1, DNMT3a, DNMT3b, HDAC1 and clinical characteristics of lung cancer was analyzed.Results：The expressions of DNMT1, DNMT3a, DNMT3b and HDAC1 in patients with lung cancer were higher than those in lung benign diseases group and control group （F=3.281,62.510,37.273,57.721,P〈0.05）.The re-sult of logistic regression showed that the overexpressions of DNMT 1, DNMT3a,DNMT3b and HDAC1 might increase the risk of lung cancer （P〈0.001）.There were no correlations among the expressions of DNMT 1, DNMT3a, DNMT3b or HDAC1 between different histological types （P〉0.05）,while there were statistical significances in the expressions of DNMT 1, DN-MT3a, DNMT3b and HDAC1 between different stages （t=0.843,0.244,0.222,0.878,P〈0.05）.Conclusion：The overex-pressions of DNMT1, DNMT3a, DNMT3b and HDAC1 could contribute to the occurrence and development of lung cancer .

关 键 词：肺 肿瘤 DNA甲基化转移酶 组蛋白去乙酰化酶1 

分 类 号：R734.2[医药卫生—肿瘤]