朱砂亚慢性染毒致大鼠肝毒性效应  

Cinnabar-induced subchronic hepatotoxicity in rats

作　　者：王大鹏[1] 王莹[1] 高显会[1] 盛禹萌 王良君[1] 王博涵[1] 吴洁[1] 马洪林[1] 

机构地区：[1]辽宁医学院公共卫生管理学院,辽宁锦州121000

出　　处：《中国公共卫生》2014年第6期743-745,共3页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(2013022056);辽宁医学院博士启动基金(Y2010B10)

摘　　要：目的探索朱砂亚慢性经口染毒对大鼠的肝毒性效应。方法将SD大鼠随机分为两组,朱砂组以每天1.0 g/kg朱砂(以0.5%羧甲基纤维素钠配成混悬液)灌胃,对照组以等体积的溶剂灌胃;分别于给药8、12 w后次日处死半数动物,取血液和肝脏,检测汞含量、血清生化指标、组织病理学。结果给药8、12 w后,朱砂组血汞含量分别为(13.843±3.928)、(14.038±4.446)μg/L,明显高于对照组(7.523±3.85)、(6.289±3.258)μg/L(P<0.05),朱砂组肝汞含量分别为(0.107±0.051)、(0.147±0.065)μg/g,明显高于对照组(0.055±0.02)、(0.045±0.028)μg/g(P<0.05);给药8 w后朱砂组AST含量为(130.17±32.36)U/L,高于对照组(95.83±17.86)U/L(P<0.05);给药12 w后总蛋白、白蛋白含量均高于对照组;镜下朱砂组可见肝细胞肿胀,空泡变性、炎性细胞浸润等病理改变。结论长期过量使用朱砂可引起肝脏汞蓄积,造成肝脏损伤,临床上应合理使用。Objective To explore the cinnabar-induced subchronic hepatotoxicity in rats by oral administration. Methods The Sprague-Dawley （SD） rats were randomly divided into cinnabar group and control group. The rats in the treatment group were administered with cinnabar by gavage at the dosage of 1.0 g/kg/d in 0. 5% carboxymethyl cellulose sodium solution and the rats in the control group were treated with the same volume of solvent, respectively. Half of the rats in each group were sacrificed after 8 and 12 weeks of the treatment and blood and liver samples were collected for the determination of mercury content,indexes of liver function and pathological examination. Results After cinnabar expo- sure, the contents of mercury in both blood and liver were significantly higher than those of the control （ blood mercury： 13.843 ±3. 929 vs. 7. 523 ± 3.85 μg/L at 8 week, P 〈 0. 05 ; 14. 038 ± 4. 446 vs. 6. 829 ± 3.258 μg/L at 12 week, P 〈 0. 05 ; liver mercury ：0. 107 ± 0. 051 vs. 0. 055 ± 0. 020 at 8 week, P 〈 0. 01 ;0. 147 ± 0. 065 vs. 0. 045 ± 0. 028μg/g at 12 week, P 〈 0. 05 ）. The concentration of aspartate aminotransferase of cinnabar group was significantly higher than that of control group at 8 week（ 130. 17 ±32. 26 vs. 95.83 ± 17.86 U/L） （P 〈0.05）. The contents of total protein and albu- min of cinnabar group were higher than those of the control group at 12 week. Pathologieal changes in the livers of cinna- bar-treated rats were observed, including hepatocyte swelling, vacuolar degeneration, and inflammatory cell infiltration. Conclusion Long-term overdose of cinnabar could cause mercury accumulation in liver and result in liver damage. Cin- nabar should be used rationally in clinical practice.

关 键 词：朱砂 汞蓄积 肝毒性 

分 类 号：R114[医药卫生—卫生毒理学]