白蛋白纳米载体的构建及其转染胰腺癌细胞潜能的可行性  

Feasibility of bovine serum albumin nanoparticle as a novel gene vector to deliver p53 gene for targeting therapy of pancreatic cancer

作　　者：朱青云[1] 潘新亭[1] 吴力群[1] 王光峰[1] 李堃[1] 韩燕[1] 滕金龙[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《山东医药》2013年第41期8-10,共3页Shandong Medical Journal

基　　金：山东省高校科技计划自筹经费项目(J12LL60)

摘　　要：目的构建牛血白蛋白纳米基因载体,观察牛血白蛋白纳米微粒的表征,并探讨其作为基因载体的可行性。方法应用去溶剂化法构建牛血白蛋白纳米载体,制备携载p53质粒的白蛋白纳米微粒(BSA-NP-p53),利用扫描电镜观察纳米微粒的大小及表面形态等特征。分别用BSA-NP-p53、空白BSA-NP转染胰腺癌细胞,利用RTPCR和Western blot检测BSA-NP-p53对胰腺癌patu8988细胞的基因转染效率。结果成功构建BSA-NP-p53,该微粒呈均匀分散的球形颗粒,粒径(115.6±12.3)nm;BSA-NP-p53能高效转染胰腺癌patu8988细胞。结论制备的白蛋白纳米微粒可作为基因转染的理想载体,并能高效转染胰腺癌细胞。Objective To construct the nano-carrier of bovine serum albumin, observe the characteristics of bovine serum albumin nanoparticles （BSA-NP） and to investigate the feasibility of BSA-NP as a novel gene vector to deliver p53 gene for targeting therapy of pancreatic cancer. Methods Desolvation method was applied to build BSA-NP, and BSA-NP carrying the p53 gene （ BSA-NP-p53 ） were prepared and then using scanning electron microscope to observe the character- istics such as size and surface morphology of the nanopartieles. BSA-NP-p53 and blank BSA-NP were respectively used to transfeet pancreatic cancer cells, and RT-PCR and Western blotting were applied to detect gene transfection efficiency of patu8988 pancreatic cancer cells treated with BSA-NP-p53. Results BSA-NP-p53 were successful constructed, the nano- particles were uniformly dispersed spherical particles, and the average particle size was （115.6 ± 12.3 ） nm. BSA-NP-p53 transfected patu8988 pancreatic cancer cells efficiently. Conclusion The prepared albumin nanoparticle can be used as an ideal carrier of gene transfection and transfect pancreatic cancer cells effectively.

关 键 词：胰腺肿瘤 纳米技术 遗传载体 血清白蛋白 牛 

分 类 号：R735.9[医药卫生—肿瘤]