红细胞分化去核因子在诱导小鼠红白血病细胞分化以及恶性逆转中的作用  

Differentiation and Malignant Suppression Induced by Mouse Erythroid Differentiation and Denucleation Factor on Mouse Erythroleukemia Cells

作　　者：赵青[1] 葛晔华[1] 周建平[1] 马静[1] 陈克铨[1] 薛社普[1] 韩代书[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所细胞生物室,北京100005

出　　处：《中国医学科学院学报》2001年第1期32-35,F003,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金!( 39670364)资助&&

摘　　要：研究小鼠红细胞分化去核分化因子 (MEDDF)在红细胞终末分化中的功能。方法构建在真核细胞中表达的重组质粒 pcDNA- MEDDF，用其转染小鼠红白血病细胞系 (MEL)。通过分析细胞的生长曲线、分裂指数及在半固体培养基中的集落形成率比较细胞的生长特性，采用 RT- PCR检测 c- myc及β-珠蛋白 (β- globin)基因的表达。结果转染 pcDNA- MEDDF与转染空载体 (pcDNA3.1)的细胞相比较，细胞的增殖率减慢、分裂指数降低、在半固体培养基中形成集落的数量减少。转染细胞的联苯胺阳性率达 32.8％。用 RT- PCR检测发现，转染细胞β-珠蛋白的表达量上升了 3.43倍， c- myc表达量下降了 66.3％。结论 MEDDF能使小鼠红白血病细胞分化并抑制其恶性变。Objective To investigate the roles of mouse erythroid differentiation and denucleation factor (MEDDF), newly cloned in our laboratory, in erythroid terminal differentiation. Methods Mouse erythroleukemia cells (MEL) were transfected with eukaryotic expression plasmid pcDNA- MEDDF. The changes of cell growth rate, mitotic index and colony- forming rate in semi- solid medium were investigated. The expressions of c- myc andβ- globin genes were analysed by semi- quantitative RT- PCR. Results MEL cells transfected with pcDNA- MEDDF showed significant lower growth rate, mitotic index, and colony- for- ming rate in semisolid medium(P< 0.01). The percentage of benzidine- positive cells was 32.8％ after transfection. The expression ofβ- globin in cells transfected with pcDNA- MEDDF was 3.43 times higher than that of control (MEL transfected with blank vector, pcDNA3.1), and the expression of c- myc was decreased by 66.3％ . Conclusions MEDDF can induce differentiation of MEL cell, and suppress its malignancy likely.

关 键 词：小鼠 红细胞分化去核因子 红细胞分化 红白血病 

分 类 号：R733.73[医药卫生—肿瘤]