叶酸在体外对人肝癌细胞株生长的影响  

Studies on In Vitro Inhibitory Effects of Folic Acid On Human Hepatic Cancer Cell Line

作　　者：杨小珊[1] 曾令福[1] 

机构地区：[1]华西医科大学公共卫生学院,成都610041

出　　处：《中国公共卫生》2001年第3期224-225,共2页Chinese Journal of Public Health

摘　　要：目的　研究叶酸的抑癌防癌作用 ,用叶酸在体外对人肝癌细胞SMMC - 772 1进行处理。方法　采用生长曲线法、MTT法研究叶酸对细胞的增殖和功能的影响 ;采用FCM进行细胞周期分析研究叶酸对细胞凋亡的影响。结果　提示在叶酸浓度达 13 5× 10 -5mol/L和 40 5× 10 -5mol/L时对人肝癌细胞株的增殖和功能有抑制作用 ,40 5× 10 -5mol/L时引起的凋亡率可达 2 7 3 %。结论　叶酸对脐静脉内皮细胞无抑制作用 ,表明叶酸对正常人体细胞无不良影响而对癌细胞有抑制作用。Objective To study the antitumor activities of folic acid,Folic acid are dealed with human hepatic cancer cell line SMMC-7721 in vitro.Methods By means of there approaches as follows:cell proliferation curve analysis,MTT assay and cell cycle analysis by FCM.Results That after treatment with 13.5×10\+\{-5\}mol/L,40.5×10\+\{-5\}mol/L folic acid selectively inhibited the activity and function of SMMC-7721.There were apoptotic peaks in cell cycle analysis by FCM and apoptosis rate reached 27.1%.Conclusion The effects of folic acid on human umbilical vein end othelial cells were slightly.

关 键 词：肝癌细胞株 叶酸 抑癌作用 细胞凋亡 实验研究 

分 类 号：R73－36[医药卫生—肿瘤] R735.7[医药卫生—临床医学]