Cy5标记寡脱氧核苷酸MT01经大鼠牙龈黏膜局部注射后在重要脏器组织中的分布  

Distribution of Cy5-labeled oligodexynucleotide MT01 in main organ tissues after local injection in gingival mucosa of rats

作　　者：张超[1] 申玉芹[2] 侯旭[1] 裴瑾[3] 孙晗[1] 孙新华[1] 

机构地区：[1]吉林大学口腔医院正畸科,吉林长春130021 [2]吉林大学口腔医院牙周病科,吉林长春130021 [3]吉林大学药学院生物化学与分子生物学,吉林长春130021

出　　处：《吉林大学学报（医学版）》2014年第3期574-577,I0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省发展和改革委员会产业技术研究与开发项目资助课题(JF2012C009-4)

摘　　要：目的:观察不同时间点Cy5标记寡脱氧核苷酸(ODN)MT01在大鼠体内重要脏器组织中的分布,初步探讨其分布的规律性。方法:选取60只Wistar大鼠随机分为实验组和对照组,每组30只;实验组大鼠牙龈黏膜局部注射Cy5标记的MT01,对照组大鼠牙龈黏膜局部注射未经标记的MT01;于注射后15min、1h、4h、8h、16h、1d、2d、3d、4d和5d分别取大鼠肺、肝、脾、肾、心和脑组织;激光共聚焦显微镜下观测大鼠重要脏器组织中MT01的荧光分布,以荧光阳性细胞率表示各脏器组织内MT01进入各组织细胞量。结果:对照组大鼠重要脏器组织中未观察到荧光阳性细胞;实验组大鼠除心、脑组织外,肺、肝、脾和肾组织细胞中均可观察到荧光阳性细胞;肾组织中荧光阳性细胞呈片状分布,荧光主要集中于肾小管上皮细胞的胞质中;肝、脾和肾组织的荧光阳性细胞率呈规律性变化,峰值分别出现在注射后4、3和4d时,肺组织的荧光阳性细胞率未见明显规律性。结论:MT01经大鼠牙龈局部注射后可进入肺、肝、脾和肾组织,主要集中在肾脏组织中,且分布具有一定规律性。Objective To observe the distribution of oligodexynucleotide （ODN）MT01 in main organ tissues of the rats at different time points and to discuss the regularity of the distribution of MT01 preliminarily. Methods 60 male Wistar rats were randomly divided into experimental group（n=30）and control group（n=30）. The rats in experimental group was locally injected with Cy5 labeled MT01 in gingival mucosa,whereas the rats in control group were injected with MTO1.The samples of rat lung,liver spleen,kidney,heart,and brain tissues were collected at 15 min,1 h,4 h,8 h,16 h,1 d,2 d,3 d,4 d,and 5 d after injection,and the distribution of MT01 fluorescence was observed by laser scanning confocal microscope.The ratio of fluorescence positive cells indicated the amount of MT01 that had been taken up by different organs.Results No positive fluorescence cells were observed in control group.Whereas,in experimental group ,the positive fluorescence cells were detected in＆amp;nbsp;the tissue samples of lung,liver,spleen and kidney but not in the tissue samples of heart and brain.The positive fluorescence cells distributed focally in kidney tissue and presented primarily in the cytoplasm of renal tubular epithelial cells.The ratios of positive fluorescence cells changed regularly with time in liver, spleen and kidney tissues and the highest level was detected at 4,3 and 4 d after injection.No distinct regularity of the ratio of positive fluorescence cells was observed in lung tissue.Conclusion MT01 can be taken up by liver,spleen and lung tissue and primarily by kidney with regularity in distribution.

关 键 词：寡脱氧核苷酸 MT01 Cy5标记 大鼠 WISTAR 

分 类 号：R363[医药卫生—病理学]