口腔鳞状细胞癌C-erbB-2蛋白表达基因扩增及其临床意义  

The expression of C-erbB-2 protein,gene amplification and its clinical significance in oral squamous cell carcinoma(OSCC)

作　　者：邓巍[1] 谢洪[1] 孙晓菊[1] 

机构地区：[1]辽宁省人民医院口腔科,沈阳110016

出　　处：《中国实用口腔科杂志》2014年第5期290-293,共4页Chinese Journal of Practical Stomatology

摘　　要：目的探讨C-erbB-2在口腔鳞状细胞癌(OSCC)中的蛋白表达及基因扩增,并评估其在OSCC发生、发展和预后中的价值。方法收集2003年1月至2013年6月辽宁省人民医院手术切除并经病理确诊为OSCC的石蜡包埋组织蜡块60例及正常口腔黏膜组织蜡块30例,分别采用免疫组织化学SP法与荧光原位杂交(FISH)技术检测C-erbB-2蛋白表达及基因扩增。结果 C-erbB-2蛋白在OSCC、正常口腔黏膜组织中的表达阳性率分别为31.7%、3.3%;扩增阳性率分别为28.3%、0;OSCC中C-erbB-2蛋白表达、基因扩增与正常口腔黏膜组织相比较,差异具有统计学意义(P<0.05);在OSCC组织中C-erbB-2蛋白表达、基因扩增与患者的年龄、性别、肿瘤大小、分化程度均无相关性(均P>0.05),与肿瘤淋巴结转移、远处转移、临床分期相关(χ2=10.808、17.100、4.627,χ2=8.840、20.044、5.102,均P<0.05)。结论 C-erbB-2与OSCC的发生、发展有关,可以将其作为判断OSCC的生物学行为的重要参考指标。Objective To investigate the expression of C-erbB-2 protein and gene amplification in OSCC and to evaluate C-erbB-2 in OSCC occurrence, development and prognosis. Methods Use immunohistochemical SP method and in situ chemical hybridization （FISH）to detect expression of C-erbB-2 protein and gene amplification in 60 cases of OS- CC and 30 cases of normal oral mucosa in paraffin-embedded tissue chip material. Results The positive rate of C-erbB-2 expression in OSCC and normal oral mucosa tissues was 31.7% and 3.33% respectively. The positive rate of C-erbB-2 gene amplification was 28.3% and O.There were significant differences between OSCC and oral mucosa tis- sues in the expression and amplification of C-erbB-2.The expression and amplification of C-erbB-2 in OSCC had no correlation with age, gender or tumor differentiation degree of patients （P 〉 0.05）, but were correlated with the lymph node metastasis, distant metastasis and clinical stage （P 〈 0.05）.Conclusion C-erbB-2 is closely related to the occurrence and development of OSCC, and can be considered as an important index for determing the biological behavior of OSCC.

关 键 词：口腔鳞状细胞癌 C-ERBB-2 免疫组织化学 荧光 

分 类 号：R78[医药卫生—口腔医学]