组织薄片法在人原代肝癌细胞培养中的应用  

The application of the primary culture of liver cancer cells with tissue slice method

作　　者：林满洲[1] 胡敏[1] 吴桂林[1] 缪辉来[1] 朱润芝[2] 黄海丽[3] 李明意[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524000 [2]广东医学院肝胆外科研究室 [3]广东医学院临床医研究中心

出　　处：《岭南现代临床外科》2014年第3期239-243,共5页Lingnan Modern Clinics in Surgery

基　　金：广东省科技项目(2008B030301028);广东医学院科技创新基金(STIF201107)

摘　　要：目的探讨组织薄片法在人原代肝癌细胞培养中的应用。方法分别运用组织薄片法和组织块法从人肝癌组织标本中分离培养原代肝癌细胞,分析两种方法培养成功率、细胞爬出时间、形态及GPC3的表达。结果薄片法培养成功率为66.67%,爬出细胞时间为(7.89±0.78)d,在培养第一代就能观察到形态规则的细胞且周围培养环境洁净;组织块法培养成功率为61.11%,爬出细胞时间为(7.56±0.73)d,在培养第二代能观察到形态规则的细胞。同时原代肝癌细胞和HepG2均能稳定表达GPC3。结论两种方法均能成功培养出原代肝癌细胞,但薄片法培养的细胞在形态及洁净程度上较组织块好,同时能稳定表达GPC3。Objective To investigate the application of the primary culture of liver cancer cells with tissue slice method. Methods The primary liver cancer cells from human liver tissue were isolated by using tissue slice method and tissue pieces respectively, and the success rate of cultivation, the cell climbed-out time, cell morphology, and the expression of GPC3 of two methods were analyzed. Results The success rate of cultivation with the tissue slice method was 66.67% ,the cell climbed-out time was （7.89 ±0.78） d. In the second generation of culture cells can be observed with morphological rules. Meanwhile HepG2 cells and primary liver cancer stably expressed GPC3. Conclusion The both methods can successfully cultured primary liver cancer ceils, but it is better with tissue slice method to raise the liver cancer cells in the cell morphology and cleanliness and which can stably express GPC3

关 键 词：组织薄片法 人肝癌组织 GPC3 爬出细胞时间 形态 

分 类 号：R657.3[医药卫生—外科学] R73-35[医药卫生—临床医学]