响应曲面优化超微粉碎参须中人参总皂苷的溶出工艺  

Optimization of Dissolution Process for Superfine Grinding Technology on Total Saponins of Panax ginseng Fibrous Root by Response Surface Methodology

作　　者：赵亚[1] 赖小平[1] 姚海燕[1] 赵冉[1] 吴依娜[1] 李耿[1] 

机构地区：[1]广州中医药大学,广东广州510006

出　　处：《中药材》2014年第3期494-498,共5页Journal of Chinese Medicinal Materials

基　　金：广州市白云区科技计划(2009-SZ-44;2011-K2-103)

摘　　要：目的:研究超微粉碎的粉碎度、提取时间、乙醇体积分数、提取温度等因素对参须中人参总皂苷溶出的影响,并探讨不同因素之间的交互作用。方法:通过单因素试验探讨粉碎度、提取时间、乙醇体积分数、提取温度等因素对参须中人参总皂苷溶出率的影响,在单因素试验基础上,利用响应曲面法对影响人参总皂苷溶出率的3个主要因素即超微粉碎时间、提取时间和乙醇体积分数进行优化。结果:人参总皂苷的含量与所考察的3个因素之间关系符合二次模型。确定最佳溶出工艺参数为:参须超微粉碎时间为9 min,70%乙醇为溶剂,提取温度50℃,提取时间70 min。实验结果显示,此条件下人参总皂苷平均溶出率为94.81%,与模型预测值非常接近,说明该模型比较可靠。结论:该优化工艺溶出快速、高效、简单、稳定。Objective：To investigate the effects of superfine cornminution extraction technology of ginseng total saponins from Panax ginseng fibrous root, and to make sure the optimal extraction condition. Methods ： Optimal condition of ginseng total saponins from Panax ginseng fibrous root was based on single factor experiment to study the effects of crushing degree, extraction time, alcohol concen- tration and extraction temperature on extraction rate. Response surface method was used to investigate three main factors such as superfine eomminution time, extraction time and alcohol concentration. Results：The relationship between content of ginseng total saponins in Panax ginseng fibrous root and three factors fitted second degree polynomial models. The optimal extraction condition was 9 rain of superfine eomminution time ,70% of alcohol ,50 ℃ of extraction temperature and 70 min of extraction time. Under the optimal condition, ginseng total saponins from Panax ginseng fibrous root was average 94. 81% , which was consistent with the predicted value. Conclusion ： The optimization of technology is rapid, efficient, simple and stable.

关 键 词：参须 人参总皂苷 响应曲面法 超微粉碎 

分 类 号：R284.2[医药卫生—中药学]