p73基因及其蛋白在鼻咽癌中表达的临床意义  

Clinical significance of expression of p73 gene and its protein in nasopharyngeal carcinoma

作　　者：罗海清[1] 杨东红[1] 余忠华[1] 李祥勇[2] 

机构地区：[1]广东医学院附属医院肿瘤中心二区,广东湛江524001 [2]广东医学院生物化学与分子生物学教研室

出　　处：《肿瘤研究与临床》2014年第5期318-321,共4页Cancer Research and Clinic

基　　金：基金项目：湛江市科技计划（2010C3107010）

摘　　要：目的分析p73基因及其蛋白在鼻咽癌组织中的表达及其与临床病理特征的相关性。方法采用实时荧光定量PCR（RQ-RT-PCR）法检测52例鼻咽癌组织和25例正常鼻咽组织p73基因表达水平，采用免疫组织化学染色法检测p73蛋白的表达水平，并分析它们与各项临床病理指标的相关性。结果52例鼻咽癌组织中p73基因表达阳性38例（73．1％），蛋白阳性表达37例（71．2％）；鼻咽正常组织中p73基因表达阳性6例（24．0％），蛋白阳性表达9例（36．0％）。p73基因及其蛋白在鼻咽癌组织中的表达均高于正常鼻咽组织，差异均有统计学意义（均P〈0．05），且p73基因及其蛋白的表达与癌组织的浸润深度、病理分化程度、临床分期密切相关（P〈0．05），与患者的年龄、性别无关（P〉0．05）。结论检测p73基因及其蛋白的表达将对鼻咽癌的诊断、预后判断具有重要的临床意义。Objective To investigate the expression of p73 gene and its protein and their relation with clinicopathologic features in nasopharyngeal carcinoma （NPC） tissues. Methods Expression of p73 mRNA and protein in 52 NPC and 25 normal nasopharyngeal tissues was detected by real-time fluorescent quantitative PCR and immunohistochemistry. Results Expression of p73 mRNA and protein was significantly higher in NPC than that in normal nasopharyngeal tissues （mRNA： 73.1% vs 24.0 %, protein： 71.2 % vs 36.0 %）, there were significant statistical differences between the two groups （P 〈 0.05）, and their expression was closely related to tumor invasion depth, degree of differentiation and clinical stage （P 〈 0.05）. Expression of p73 gene and protein was not closely related to age and gender （P 〉 0.05）. Conclusion Detection expression of p73 mRNA and its protein can be helpful in the diagnosis and prognostic evaluation in NPC.

关 键 词：鼻咽肿瘤 基因 P73 蛋白 

分 类 号：R739.63[医药卫生—肿瘤]