告达庭激活Caspase-3诱导人胃癌细胞SGC-7901凋亡  

Caudatin Induced Apoptosis of Gastric Cancer Cell Line SGC-7901 by Activation of Caspase-3

作　　者：朱赴东 周霞[2] 陶雪芬[3] 杨波[3] 王一奇[3] 

机构地区：[1]浙江省口腔医院,浙江杭州310006 [2]浙江省肿瘤医院,浙江杭州310022 [3]浙江中医药大学药学院,杭州310053

出　　处：《中国肿瘤》2014年第6期523-527,共5页China Cancer

基　　金：浙江省自然基金项目(LQ12H28004);浙江省医药卫生科技计划(2007QN011;2010Z006);浙江省医药卫生科技计划(2011KYB024)

摘　　要：[目的]评价告达庭体外诱导人胃癌细胞SGC-7901凋亡的作用。[方法]MTT法检测细胞活性;吖啶橙染色观察核形态;流式细胞术检测细胞凋亡率;Western-blot及比色法检测Caspase-3表达及活性。[结果]告达庭抑制SGC-7901细胞的活性,具有浓度、时间依赖性;告达庭70μM能使SGC-7901细胞出现核染色体浓聚等典型的凋亡形态学改变,告达庭35、70μM使细胞凋亡率升高,并使Caspase-3蛋白表达量和蛋白活性显著升高。[结论]告达庭可能通过激活Caspase-3诱导SGC-7901细胞凋亡。[ Purpose ] To investigate the apoptosis inducing effects and molecular mechanisms of Cau- datin in gastric cancer cell SGC-7901. [Methods] SGC-7901 cells were cultured in vitro and treated with various concentrations of Caudatin. Cell proliferation was examined by MTr assay and apoptosis were detected by Flow cytometry. Morphological changes were detected by acridine orange staining. The protein expression of caspase-3 was detected by Western blot analysis and the activity of cas- pase-3 was detected by Colorimetric assay. [Results] Caudatin inhibited the growth of SGC-7901 cells in a time and concentration-dependent manner. Caudatin could also induce cell nuclear mor- phological changes,and increase the expression and activity of caspase-3.[Conclusion] Caudatin may inhibit cell proliferation and induce the apoptosis of SGC-7901 by activating caspase-3.

关 键 词：告达庭 胃癌 凋亡 CASPASE-3 SGC-7901 

分 类 号：R735.2[医药卫生—肿瘤]