凋亡素2配体对放射线诱导95-D细胞凋亡的影响的研究  

Study on the Effects of Opoptosis-2 Ligand on Apoptosis of 95-D Lung Cancer Cells that Radiation Induced

作　　者：韩蕃颉 张红军[1] 刘希光[1] 宋浩[1] 冯龄鑫[1] 王相[2] 

机构地区：[1]青岛大学附属医院肿瘤科,山东青岛266003 [2]青岛大学附属医院放疗中心,山东青岛266003

出　　处：《现代生物医学进展》2014年第17期3244-3247,共4页Progress in Modern Biomedicine

基　　金：卫生部科研课题(W2012FZ078)

摘　　要：目的:探讨凋亡素2配体(Apo-2L)对放射线诱导肺癌95-D细胞凋亡的影响。方法:MTT法检测不同浓度凋亡素2配体在体外对肺癌95-D细胞的抑制率,将细胞分为4组,对照组、凋亡素2配体组、单纯照射组、凋亡素2配体+放射照射组,流式细胞仪检测各组凋亡率及细胞周期。结果:凋亡素2配体对95-D细胞的体外抑制作用明显,随着药物浓度的增大及时间的延长,抑制率明显增高(P<0.05)。流式细胞术显示凋亡素2配体与放射线联用能够使95-D细胞的凋亡率提高,与单用凋亡素2配体组及单纯放疗组相比,凋亡率差异显著(P<0.05)。结论:凋亡素2配体在体外具有抑制95-D细胞增殖的作用并能够促进细胞的凋亡,同时凋亡素2配体联合放射线可以明显提高肺癌95-D细胞的凋亡率。Objective： To investigate the effect of opoptosis-2 ligand on apoptosis of 95-D cells that radiation induced. Methods： The effect of the different concentration of the apoptosis-2 ligand on cellular proliferation inhibition rate of 95-D cells was detected by MTT method; The effect of combined apoptosis-2 ligand with radiation on the cell apoptosis and the cell cycle was detected by flow cytometry. Results： The cellular proliferation inhibition rate of apoptosis-2 ligand on 95-D cells was related with the drug concentration and action time （P〈0.05）. The combined application of apoptosis-2 ligand and radiation could raise the cellular proliferation inhibition rate of 95-D cells, compared with that in the control group and apoptosis-2 ligand or radiation alone（P〈0.05 ）. Conclusions： Apoptosis-2 ligand can inhibit the proliferation of 95-D cells in vitro culture and combined with apoptosis-2 ligand and radiation can raise the cellular proliferation inhibition rate of 95-D cells.

关 键 词：凋亡素2配体 细胞凋亡 细胞增殖 放射线疗法 

分 类 号：R734.2[医药卫生—肿瘤]