结肠炎相关结肠癌分泌蛋白质体外富集方法的建立  

Establishment of in Vitro Methods for Collection of Colitis-associated Colon Cancer Secretome

作　　者：马怡茗[1] 肖汀[1] 赵新华[1] 韩文晓[1] 汪红英[1] 

机构地区：[1]北京协和医学院中国医学科学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《现代生物医学进展》2014年第17期3259-3261,共3页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展规划项目(2011CB910700);国家自然科学基金重大研究计划培育项目(91129717)

摘　　要：目的:建立小鼠结肠炎相关结肠癌分泌蛋白质体外富集的方法;方法:利用结肠炎相关结肠癌小鼠模型,取不同阶段实验小鼠的结肠体外培养48h,收集分泌蛋白质。使用SDS-PAGE分离分析分泌蛋白质,并通过Western blot检测分泌蛋白质是否存在胞浆蛋白或者核蛋白的污染。结果:在实验条件下收集得到的分泌蛋白重现性好,分泌蛋白中核蛋白和胞浆蛋白的污染较少,且能够明显分辨与病程相关差异表达蛋白质条带。结论:成功建立了结肠炎相关结肠癌分泌蛋白质的体外富集方法。Objective： To establish in vitro method to collect the secreted proteins from mouse with colitis-associated colon can- cer. Methods： Colitis-associated colon cancer was induced by azoxymethane （AOM） and dextran sulfate sodium （DSS） in C57BL/6 mice. Colon tissue was collected at different stages during the development of colon cancer. The whole colons were sectioned into pieces for in vitro culture with semm-fi＇ee media, and then the conditioned media were collected at 48h. Western blot was applied to detect whether there is cytoplasm and nucleus protein contaminant in secreted proteins. Results： The method had good reproducibility. There was trace amount of contaminants from cytoplasm and nucleus in collected secretome. And several different protein bands were observed among different groups and correlated with the stage of colitis-associated colon cancer. Conclusion： An in vitro method to collect secreted pro- teins from mouse with colitis-associated colon cancer was successfully established.

关 键 词：结肠炎相关结肠癌 分泌蛋白质 组织培养 

分 类 号：R735.35[医药卫生—肿瘤] Q5[医药卫生—临床医学]